[No public or meaningful name is available]

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24th January 2012 to 19th June 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

no

Remarks:

The study was not within the scope of regulations governing the conduct of noncliniical laboratory studies and is not intended to comply with the regulations

Test type:

acute toxic class method

Test material

Test material information

Constituent 1

Reference substance name:

{1-(ETHYLSULFONYL)-3-[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)-1H-PYRAZOL-1-YL]AZETIDIN-3-YL}ACETONITRILE

Cas Number:

191987-50-5

Molecular formula:

C16H25BN4O4S

IUPAC Name:

{1-(ETHYLSULFONYL)-3-[4-(4,4,5,5-TETRAMETHYL-1,3,2-DIOXABOROLAN-2-YL)-1H-PYRAZOL-1-YL]AZETIDIN-3-YL}ACETONITRILE

Test material form:

solid: particulate/powder

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Animals were received on 24 Jan 2012, 14 Feb 2012 and 28 Feb 2012.
Following an acclimation period of at least five days, six healthy male and six healthy, non-pregnant and nulliparous female Sprague Dawley rats were assigned to treatment groups without conscious bias.
The animals were born the weeks of 22 Nov 2011, 29 Nov 2011, 20 Dec 2011 and 17 Jan 2012. The pretest body weight range was 192 - 408 grams for males and 184 - 227 grams for females.
The animals were identified by cage notation and indelible body marks, and housed in suspended wire cages, five per sex per cage prior to dosing and three per sex per cage following dosing. Absorbent paper bedding was placed beneath the cages and changed at least three times per week. Fresh PMI Rat Chow (Diet #5012) was freely available except for 16-20 hours prior to dosing. Water was available ad libitum. The animal room, reserved exclusively for rats on acute tests, was temperature controlled, had a 12-hour light/dark cycle, and was kept clean and vermin free.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

methylcellulose

Remarks:

2%

Details on oral exposure:

The test article was mixed with 2% methylcellulose solution to make dosing by gavage possible. The dose was based on the dry weight of the test article. A single dose was administered orally by syringe and dosing needle at a dose level of 300 mg/kg to three three female and three male rats and at a dose level of 2000 mg/kg to three female and three male rats.

Doses:

300 mg/kg to three three female and three male rats.
2000 mg/kg to three female and three male rats.

No. of animals per sex per dose:

3

Control animals:

no

Details on study design:

Type and Frequency of Observations
In Vivo - The rats were observed at 15 minutes, 1, 2 and 4 hours post dose and daily for 14 days for toxicity and pharmacological effects. Animals were observed twice daily for mortality. Body weights were recorded immediately pretest, weekly and at termination.
Post Mortem – All animals were humanely sacrificed using CO2 and were examined for gross pathology following study termination.

Statistics:

An estimate of the LD50 was made based on mortality occurring during the study.

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

There were no deaths at either the 300mg/kg or 2000mg/kg dose rates.

Clinical signs:

No abnormal physical signs were observed.

Body weight:

All of the animals gained body weight.

Gross pathology:

The gross necropsy of all animals revealed no observable abnormalities

Applicant's summary and conclusion

Interpretation of results:

Category 5 based on GHS criteria

Conclusions:

Compound 3030577 is considered to be in Acute Toxic Category 5, or unclassified. The oral LD50 is greater than 2000 mg/kg of body weight in rats.

Executive summary:

Objective: To determine the potential for oral toxicity using the Acute Toxic Class Determination. This study was designed to comply with the standards set forth in OECD Guidelines for the Testing of Chemicals, Guideline 423 adopted December 17, 2001. The test article was assigned to a toxic category based on the mortality results and significant clinical signs of toxicity up to Category 4 value tested according to Globally Harmonized System of Classification and Labeling of Chemicals (GHS). Third Revised Edition. United Nations - New York and Geneva. 2009.

Method Synopsis: Initially, three healthy female Sprague Dawley rats were dosed orally with Compound 3030577, Lot# HB4-H71167-068 at 300 mg/kg. An additional three healthy males were dosed as a confirmatory group at 300 mg/kg. Since there was no toxicity noted at this level, an additional three healthy female and three healthy male Sprague Dawley rats were dosed orally at 2000 mg/kg. The rats were observed at 15 minutes, 1, 2 and 4 hours post dose and daily for 14 days for toxicity and pharmacological effects. Animals were observed twice daily for mortality. Body weights were recorded immediately pretest, weekly and at termination. All animals were examined for gross pathology. The test article was assigned to a toxic category based on the mortality response noted.

Summary: 

300 mg/kg: Three females and three males survived the single 300 mg/kg oral dose. There were no abnormal physical signs were observed. All of the animals gained body weight. The gross necropsy of all animals revealed no observable abnormalities.
2000 mg/kg: Three females and three males survived the single 2000 mg/kg oral dose. There were no abnormal physical signs were observed. All of the animals gained body weight. The gross necropsy of all animals revealed no observable abnormalities.

Conclusion: Compound 3030577, Lot# HB4-H71167-068 is considered to be in Acute Toxic Category 5, or unclassified. The oral LD50 is greater than 2000 mg/kg of body weight in rats.