(Z)-non-6-enal

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

April 16-July 1, 2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2020

Report date:

2020

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

(Z)-non-6-enal

EC Number:

218-900-9

EC Name:

(Z)-non-6-enal

Cas Number:

2277-19-2

Molecular formula:

C9H16O

IUPAC Name:

(Z)-non-6-enal

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all concentrations
- Sample storage conditions before analysis: Samples were placed in glass vials with formic acid, and then diluted with Elendt M4 medium to 5 mL. 50 uL of internal standard was then added, and the sample extracted with hexane.

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to brown sterile bottles and Elendt M4 medium was added with a sterilized stir bar. The test solution was then stirred for 72 hrs. It was then allowed to settle for 1 hr. Since the test substance had a higher density than water, it settled to the bottom of the flask. The settled test substance in the bottom 200 mL was drawn off, and the test solution taken from the higher water layer saturated with test substance.
- Evidence of undissolved material (e.g. precipitate, surface film, etc.): At higher concentration some precipitate may have formed.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Age at study initiation (mean and range, SD): 4-24 hrs old
- Source: in-house
- Age of parental stock (mean and range, SD): 3 weeks
- Feeding during test : None

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

None

Test conditions

Test temperature:

18-22 degrees C

pH:

7.72-8.56

Dissolved oxygen:

8.33-8.97 mg/L

Nominal and measured concentrations:

Nominal: 1.05, 2.32, 5.10, 11.2, 24.7 mg/L
Measured:

Details on test conditions:

TEST SYSTEM
- Test vessel: Conical flasks
- Type (delete if not applicable): covered with air-permeable silicone sponge caps
- Material, size, headspace, fill volume: 100 mL
- Volume of solution: 100 mL
- Aeration: None
- Renewal rate of test solution (frequency/flow rate): 24 hrs
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 5

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4 medium

OTHER TEST CONDITIONS
- Adjustment of pH: 7.72-8.45
- Light intensity: 833-841 Lux

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): immobility at 0, 24, and 48 hrs.

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

9.06 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.07 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mobility

Details on results:

- Behavioural abnormalities: At 48 hrs the daphnids in the 8.16 mg/L concentration were less mobile than controls.
- Mortality of control: None
- Immobilisation of control: None

Reported statistics and error estimates:

95% confidence limit of EC50: 7.14-11.3 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 48-hr EC50 for daphnids was 9.06 mg/L.

Executive summary:

An OECD Guideline 202 aquatic invertebrate toxicity study was performed. Five daphnids were exposed to each concentration, with four replicates per concentration. The measured concentrations tested were below the limit of quantification, 1.20, 3.07, 8.05, and 18.44 mg/L. Daphnids at the 8.05 mg/L concentration showed less activity than at lower concentrations or controls. The NOEC was determined to be 3.07 mg/L based on active ingredient. The EC50 was determined to be 9.06 mg/L.