N1,N2-dimethyl-N1-{2-[methyl(propan-2-yl)amino]ethyl}-N2-(propan-2-yl)ethane-1,2-diamine

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2020

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Version / remarks:

OECD 301 F (July 1992)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Active substance: 100%

Oxygen conditions:

anaerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

Activated sludge from the municipal wastewater treatment plant Breisgauer Bucht was used as inoculum with a concentration corresponding to 30 mg dry solids per litre. The treatment plant clarifies predominantly domestic wastewater and has a capacity of 600.000 inhabitant equivalents. Sampling date of activated sludge was 21 January 2020. The dry solids (d.s.) content of the activated sludge was 4.6 g/L. It was determined by weight measurements after drying at 105°C for about 4.5 hours (mean of triplicate measurements).

Duration of test (contact time):

28 d

Initial conc.:

60.1 mg/L

Based on:

ThOD

Key result

Parameter:

% degradation (TOC removal)

Value:

0

Sampling time:

28 d

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

No degradation of the test item could be observed within the test duration. The mean degradation after 28 days was just below 0% (-0.1% ThODNO3 / ThODNH4, each mean of three replicates).
The test item did not reach the criteria for ready biodegradability (60% of ThOD within 28 days and within a 10-d window).
The degradation of the toxicity control after 14 days was >25%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Executive summary:

No degradation of the test item could be observed within the test duration. The mean degradation after 28 days was just below 0% (-0.1% ThODNO3 / ThODNH4, each mean of three replicates).

The test item did not reach the criteria for ready biodegradability (60% of ThOD within 28 days and within a 10-d window).

The degradation of the toxicity control after 14 days was >25%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Description of key information

No degradation of the test item could be observed within the test duration. The mean degradation after 28 days was just below 0% (-0.1% ThODNO3 / ThODNH4, each mean of three

replicates).

The test item did not reach the criteria for ready biodegradability (60% of ThOD within 28 days and within a 10-d window).

The degradation of the toxicity control after 14 days was >25%. The test item had no inhibitory effect on the inoculum according to the criterion of the guideline.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater