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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
3 hours
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Guideline study 2018 to GLP
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)
Specific details on test material used for the study:
Physical state/Appearance: pale straw colored liquid
Purity: >98%
Analytical monitoring:
not required
Vehicle:
not specified
Details on test solutions:
Nominal test concentrations of 10, 100 and 1000 mg/L. The test item was dispersed directly in the water-based test system
Test organisms (species):
activated sludge of a predominantly domestic sewage
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Test temperature:
20 - 22 C
pH:
7.3 - 7.9 after buffering (substance alkaline)
Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol
Duration:
3 h
Dose descriptor:
EC0
Effect conc.:
> 100 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significantly toxic effects (P < 0.05) were shown at the test concentration of 1000 mg/L. Based on these results the No Observed Effect Concentration (NOEC) after 3 hours exposure was 100 mg/L.
Validity criteria fulfilled:
yes
Conclusions:
No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significantly toxic effects (P < 0.05) were shown at the test concentration of 1000 mg/L. Based on these results the No Observed Effect Concentration (NOEC) after 3 hours exposure was 100 mg/L.

Description of key information

No statistically significant toxic effects were shown at the test concentrations of 10 and 100 mg/L, however statistically significantly toxic effects (P < 0.05) were shown at the test concentration of 1000 mg/L. Based on these results the No Observed Effect Concentration (NOEC) after 3 hours exposure was 100 mg/L.

Key value for chemical safety assessment

EC50 for microorganisms:
1 000 mg/L

Additional information

Figure used for CSA; EC50 > 1000 mg/l