Methyl 3-aminocrotonate

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Version / remarks:

not mentioned

Deviations:

not specified

Principles of method if other than guideline:

According to the department of transportation act.

GLP compliance:

no

Test material

Test material information

Constituent 1

Test material form:

solid: crystalline

Details on test material:

Colour: light yellow
Odour: Ammoniacal

Test animals

Species:

rabbit

Strain:

not specified

Test system

Type of coverage:

occlusive

Preparation of test site:

abraded

Vehicle:

unchanged (no vehicle)

Controls:

yes, concurrent no treatment

Amount / concentration applied:

0.5 g

Duration of treatment / exposure:

4, 24 and 48 h

Observation period:

24 h and 48 h

Number of animals:

6

Details on study design:

The intact and abraded skin of 6 albino rabbits was employed for this study. The hair was clipped from the backs with the aid of angora clippers. Four
areas of the back, placed approximately ten centimeters apart, were designated for the positions of the patches. Areas 2 and 3 were abraded by
making four epidermal incisions (two perpendicular to two others in the area of the patch). The patches consisted of 1. 5 inch x l. 5 inch 12 ply
gauze squares. The patches were secured to the area by thin bands of adhesive tape. The material to be tested 0.5 g was introduced beneath the patch.
The entire trunks of the animals were then wrapped in clear plastic trunk bands. The trunk bands help to hold the patches in position and retard evaporation of
volatile substances during the four hour exposure period. Upon removal of the patches the resulting reactions were evaluated on the basis of weighted
scores. Following this initial reading, all test sites were washed with appropriate solvent to prevent further exposure. Readings were again made
at 24 and 48 hours after the initial application.The test substance is evaluated on a total of site (6 abraded and 6 intact).

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

fully reversible

Results 2

Irritation parameter:

edema score

Basis:

mean

Time point:

24/48/72 h

Score:

0

Max. score:

0

Reversibility:

fully reversible

Irritant / corrosive response data:

no corrosion observed

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

In the skin irritation study no corrosion was observed.

Executive summary:

The study was performed in year 1976 according to the department of transportation act. 6 albino rabbits were used for the study. An Addendum to the report was prepared in year 1991.

Overall mean of erythema/eschar (intact skin 24 and 48 h): 0.0; overall mean of edema (intact skin 24 and 48 h): 0.0.

In conclusion, the substance is not corrosive to the skin.