Lithium tetrafluoroborate, anhydrous

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Lithium tetrafluoroborate, anhydrous

EC Number:

238-178-9

EC Name:

Lithium tetrafluoroborate, anhydrous

Cas Number:

14283-07-9

Molecular formula:

BF4.Li

IUPAC Name:

lithium tetrafluoroborate, anhydrous

Test material form:

solid: particulate/powder

Test solutions

Vehicle:

yes

Details on test solutions:

The stock solution used in the range-finding test was prepared by dissolving 1000 mg of the test item in volume completed to 1000 mL with dilution water, resulting in a concentration of 1000 mg.L-1. To assist solubilization of the solution, ultrasonic dispersion was also applied to 20 minutes.

The stock solution used in the definitive test was prepared by dissolving 300 mg of the test item in volume completed to 3000 mL with dilution water, resulting in a concentration of 100 mg.L-1. To assist solubilization of the solution, ultrasonic dispersion was also applied to 20 minutes.

The test solutions were prepared from dilutions of the stock solutions.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

The fish were obtained from supplier Aquario Way (Taquaral - Campinas/SP), maintained and acclimated under laboratory-controlled conditions. The fish were healthy and free from any visible disease and abnormalities during the maintenance and acclimation periods

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Remarks on exposure duration:

Fish in the treatment groups and control group were observed for general symptoms at 24, 48, 72 and 96 hrs after exposure and the mortality was recorded.

Test conditions

Test temperature:

21 ~ 25 °C

pH:

7.6 ~ 7.8

Dissolved oxygen:

7.9 ~ 8.1 mg/L

Nominal and measured concentrations:

6.25; 12.5; 25; 50 and 100 mg/L.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Details on results:

It was not observed any cumulative mortality responses of Danio rerio in the evaluation times of 24, 48, 72 and 96 hours for the range-finding and definitive tests (Tables 5 and 6). No mortality or sublethal effects were observed in the control group for the range-finding and definitive tests, showing that validity of the test was achieved. The 24 hours mortality of Danio rerio in each test concentration in the reference test with potassium dichromate is presented in the Table 7.

The values of 24, 48, 72 and 96-hour LC50 of Lithium Tetrafluoroborate were higher than 100 mg.L-1. The OECD guideline nº 203 (OECD, 1992) predict of a limit test at 100 mg.L-1 of test item. The 24-hour LC50 value for the reference item, potassium dichromate, was 283.74 mg.L-1 (confidence limits at 95% = 224.98 to 357.85 mg.L-1), demonstrating that the sensitivity of the fish was within the accepted range (ISO, 1982).

Applicant's summary and conclusion

Conclusions:

The 96-hour LC50 value of Lithium Tetrafluoroborate was higher than 100 mg.L-1. The limit test concentration tested, of 100 mg.L-1, not cause acute lethal effect on fish, in 96 hours of exposure period.