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EC number: 226-149-3 | CAS number: 5303-24-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08 Dec 2015 - 06 Jan 2016
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 1992
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 835.3110 (Ready Biodegradability)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Department of Health, United Kingdom
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sewage sludge micro-organisms obtained on 7 December 2015 from the aeration stage of the Severn Trent Water Plc sewage treatment plant at Loughborough, Leicestershire, UK.
- Laboratory culture: no
- Storage conditions: No storage, used on the day of collection.
- Pretreatment: The activated sewage sludge sample was washed twice by settlement and resuspension in mineral medium to remove any excessive amounts of dissolved organic carbon (DOC). The washed sample was then maintained on continuous aeration in the laboratory at a temperature of approximately 21 °C.
- Suspended solids concentraiton: 3.1 g/L - Duration of test (contact time):
- 28 d
- Initial conc.:
- 13 mg/L
- Based on:
- test mat.
- Initial conc.:
- 10 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: according to guideline
- Solubilising agent (type and concentration if used): chloroform
- Test temperature: 20-24 °C
- pH: 7.4 - 7.7
- pH adjusted: yes; adjusted to pH 7.4 ± 0.2 using diluted hydrochloric acid or sodium hydroxide solution
- Continuous darkness: yes
- Suspended solids concentration: 30 mg/L
TEST SYSTEM
- Culturing apparatus: 5 L test vessels containing 3 L of solution
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: Approximately 24 h prior to addition of the test and reference items the vessels were filled with 2400 mL of mineral medium and 29.0 mL of inoculum and aerated overnight. The test vessels were sealed and CO2-free air bubbled through the solution at a rate of 30 to 100 mL/min per vessel and stirred continuously by magnetic stirrer.
- Measuring equipment: The samples were analyzed for IC using either a Shimadzu TOC-VCSH TOC analyzer or a Shimadzu TOC-LCSH TOC analyzer. Samples (135 or 50 μL) were injected into the IC channel of the TOC analyzer. IC analysis occurs by means of the conversion of an aqueous sample to CO2 by orthophosphoric acid or 2 M HCl using zero grade air as the carrier gas. Calibration was by reference solutions of sodium carbonate (Na2CO3). Each analysis was carried out in triplicate. The pH was measured using a Hach HQ40d Flexi handheld meter.
- Details of trap for CO2 and volatile organics if used: The CO2 produced by degradation was collected in two 500 mL Dreschel bottles containing 350 mL of 0.05 M NaOH. The CO2 absorbing solutions were prepared using purified water.
- Other: On Day 28, 1 mL of concentrated hydrochloric acid was added to each vessel to drive off any inorganic carbonates formed. The vessels were resealed, aerated overnight and the final samples taken from both absorber vessels on Day 29.
SAMPLING
- Sampling frequency: Sampled on Days 0, 2, 6, 8, 10, 14, 21, 28 and 29. The second absorber vessels were sampled on Days 0 and 29.
- Sampling method: Samples (2 mL) were taken from the first CO2 absorber vessels.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, two replicates
- Abiotic sterile control: no
- Toxicity control: yes, one replicate
- Procedure control: yes, two replicates - Reference substance:
- benzoic acid, sodium salt
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 87
- Sampling time:
- 28 d
- Details on results:
- The toxicity control was degraded to 87% after 14 d. Thus, it can be assumed that the test item is not inhibitory to the inoculum (degradation > 25%).
- Results with reference substance:
- The reference substance was degraded to 76% after 28 d thus confirming the suitability of the inoculum.
- Validity criteria fulfilled:
- yes
- Remarks:
- see "Any other information incl. table" for further details
- Interpretation of results:
- readily biodegradable
Reference
Table 1: Percentage degradation
Day |
% Biodegradation |
||
Procedure control |
Test item |
Toxicity control |
|
0 |
0 |
0 |
0 |
2 |
46 |
36 |
32 |
6 |
67 |
68 |
61 |
8 |
66 |
89 |
80 |
10 |
75 |
83 |
77 |
14 |
81 |
91 |
87 |
21 |
78 |
88 |
85 |
28 |
79 |
91 |
88 |
29 |
76 |
87 |
86 |
Table 2: Validity criteria
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
Difference of extremes of replicate values of the removal of the test chemical at the plateau, at the end of the test or at the end of the 10-d window, as appropriate, is less than 20%. |
<20% |
yes |
Percentage degradation of the reference compound has reached the pass levels by day 14. |
81% |
yes |
The IC content of the test substance suspension in the mineral medium at the beginning of the test must be less than 5% of the TC, and the total CO2 evolution in the inoculum blank at the end of the test should not normally exceed 40 mg/L medium. |
1-2% |
yes |
Description of key information
Readily biodegradable: 87% after 28 d (CO2 evolution, OECD 301B)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
One experimental study is available investigating the biodegradation of octyl laurate (CAS 5303-24-2). The study was performed according to OECD 301B (GLP). Activated sludge from a sewage treatment plant treating predominantly domestic sewage was used as inoculum. Biodegradation of the test item was followed for 28 d by measuring the evolution of CO2. The study included a reference substance (benzoic acid, sodium salt) as well as a toxicity control (benzoic acid, sodium salt and test item) in order to assess if the test item is inhibitory to the inoculum. On day 28, hydrochloric acid was added to the test vessels in order to stop microbial activity and to drive off any inorganic carbon. After 28 d a biodegradation of 87% was recorded. Since the 10-day window was met the substance can be considered as readily biodegradable according to the OECD criteria. The toxicity control attained 87% after 14 d assuming that the test item is not inhibitory to the inoculum.
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