Registration Dossier
Registration Dossier
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EC number: 500-336-2 | CAS number: 157348-58-8
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to zebrafishDanio reriowas determined according to the principlesof the OECD-Guideline for Testing of Chemicals No. 203 (1992) andEU Council Regulation (EC) No. 440/2008/ Method C.1 (2008).The aim of the study was the determination of the effects of the test item on the mortality of the fish over a period of 96 hours. An acute toxicity test with thesaturated solution with a nominal loading of10.0 mg/Lwas conducted under semi-static conditions with daily renewal of the test media.Duration of the test was 96 hours. Seven test organisms were exposed to the saturated solution and the control. Water quality parameters (temperature, pH-value and oxygen-saturation) measured at 0, 24, 48, 72 and 96 hours were determined to be within the acceptable limits. The saturated solution of the test item was analytically verified via LC-MS/MS from freshly prepared test media after 0 and 72 h and from the corresponding 24 h aged test media after 24 and 96 h. The lead component with molecular formula C26H50O7was determined. The measured concentrations of the saturated solution in the fresh media (0 and 72 h) were in the range of 63 and 76% of the nominal values and in the old media (24 and 96 h) in the range of 42 and 49% of the nominal values.Therefore, all effect values were based on the nominal test item concentration of the saturated solution. All validity criteria of the test guideline were met.The threshold concentration of the test item was found to have no lethal effects on zebrafish when tested with the saturated solution with a nominal concentration of 10.0 mg/L. Hence the substance does not cause any effects up to the water solubility limit.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 10 mg/L
Additional information
LC0 >= 10 mg/L (maximum dissolved concentration of the test item)
LC50 > 10 mg/L
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