2-(Naphthalene-2'-sulphonylamino)-benzoic acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

31 December 1991 - 15 January 1992

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

A new version of the guidance was published after release of the study report.

GLP compliance:

not specified

Specific details on test material used for the study:

Test material: NSABZ (2-(Naphthalene-2'-sulphonylamino)-benzoic acid)
Date of receipt: 20 December 1991.
Description: Very light brown powder.
Storage: In original container at room temperature.
Method of preparation: Preliminary solution in 1% v/v Tween 80-dimethylformamide.

Analytical monitoring:

no

Vehicle:

yes

Details on test solutions:

Preliminary solution in 1% v/v Tween 80-dimethylformamide.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Test species: Rainbow trout (Oncorhynchus mykiss).
Length: 5.4 ± 0.5 cm.
Weight: 1.77 ± 0.33 g. Loading: 0.89 g bodyweight/litre.
Supplier: Parkwood trout farm, Kent, U.K.
Mortalities: Zero in the 7 days prior to the start of the test.
Medication: None.

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

Approximately 50 mg/L as CaCO3.

Test temperature:

14°C

Nominal and measured concentrations:

NOMINAL CONCENTRATIONS:
Preliminary test: 0.1, 1.0 and 10 mg/L.
Definitive study: 1.0 mg/L.

Details on test conditions:

Media renewal: Daily.
Test water: Dechlorinated tap water (sodium thiosulphate).
Test vessels: 20L.
Aeration: Yes.

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Please refer to the tables in "Any other information on results incl. tables".

Sublethal observations / clinical signs:

Table 1: Preliminary study.

Concentration (mg/L)	Cumulative mortality (initial population = 2)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
Solvent control	0	0	0	0
0.1	0	0	0	0
1.0	0	0	0	0
10	2	2	2	2

 

Table 2: Definitive study.

Concentration (mg/L)	Cumulative Mortality (initial population = 10)
	24 hours	48 hours	72 hours	96 hours
Control	0	0	0	0
Solvent control	0	0	0	0
1.0, Repetition 1	0	0	0	0
1.0, Repetition 2	0	0	0	0

Validity criteria fulfilled:

not specified

Conclusions:

The 96-hour LC50 value for acute toxicity to fish of 2-(Naphthalene-2'-sulphonylamino)-benzoic acid was determined to be > 1.0 mg/L.

Executive summary:

The acute toxicity to fish (Oncorhynchus mykiss) of NSABZ (2-(Naphthalene-2'-sulphonylamino)-benzoic acid) was evaluated.

A preliminary test on series of two fishes indicated that no mortality was expected for test item concentrations of 0.1 and 1.0 mg/L after a period of 4 days. Mortality was however observed for both fishes at a test item concentration of 10 mg/L.

A definitive study was carried out on series of 10 individuals with a concentration of 1.0 mg/L of test item for a period of 4 days. Two repetitions were performed. No mortality was observed.

Description of key information

The acute toxicity to fish (Oncorhynchus mykiss) of NSABZ (2-(Naphthalene-2'-sulphonylamino)-benzoic acid) was evaluated.

A preliminary test on series of two fishes indicated that no mortality was expected for test item concentrations of 0.1 and 1.0 mg/L after a period of 4 days. Mortality was however observed for both fishes at a test item concentration of 10 mg/L.

A definitive study was carried out on series of 10 individuals with a concentration of 1.0 mg/L of test item for a period of 4 days. Two repetitions were performed. No mortality was observed.

Based on these results, it was therefore concluded that the LC50 value for 2-(Naphthalene-2'-sulphonylamino)-benzoic acid is > 1.0 mg/L. As this value is higher than the 1.0 mg/L threshold for classification as Acute Toxicity Category 1 of CLP, the substance 2-(Naphthalene-2'-sulphonylamino)-benzoic acid should therefore not be classified in the aquatic acute toxicity category of the CLP regulation.

However, since no data is available for chronic aquatic toxicity for the substance 2-(Naphthalene-2'-sulphonylamino)-benzoic acid, application of the CLP criteria leads to a classification of this substance as Toxic to aquatic life with long lasting effects (Aquatic Chronic 2).