Reaction mass of Disodium decyl(sulphonatophenoxy)benzenesulphonate and Disodium oxybis[decylbenzenesulphonate]

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1978

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: The study was not conducted according to guideline/s and GLP but the report contains sufficient data for interpretation of study results.

Reason / purpose for cross-reference:

reference to same study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

no guideline followed

Principles of method if other than guideline:

Standard static acute daphnid toxicity test

GLP compliance:

no

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
No data

Analytical monitoring:

no

Details on sampling:

No data

Vehicle:

no

Details on test solutions:

The concentrations are reported as 100% active.
The Daphnia were exposed for 48 hours in dechlorinated Lake Huron water at 17C.

Test organisms (species):

Daphnia magna

Details on test organisms:

No additional information

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

None

Hardness:

No data

Test temperature:

17C

pH:

No data

Dissolved oxygen:

No data

Salinity:

No data

Nominal and measured concentrations:

Nominal- 0.56, 1.00, 1.80, 3.20, 5.60, and 10.0 mg/L (based on active ingredient)

Details on test conditions:

The Daphnia were exposed for 48 hours in dechlorinated Lake Huron water at 17C.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

1.5 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

mortality

Remarks on result:

other: 95% CL: 1.24-1.80 mg/L

Details on results:

Static Acute Daphnia magna Toxicity in Static Water - 48 Hours
No Kill Level <0.56 mg/L
Partial Kill 0.56 mg/L
100% Kill 5.60 mg/L

Calculated LCx Data (95% confidence limits are in parentheses after value)
LC 10 0.59 mg/L (0.39-0.77)
LC 50 1.50 mg/L (1.24-1.80)
LC 90 3.78 mg/L (2.96-5.49)
Slope* 3.19 (2.34-4.03)

*Slope represents the rate of change in mortality with concentration on a logarithmic scale.

Results with reference substance (positive control):

None

Reported statistics and error estimates:

No data

None

Validity criteria fulfilled:

not specified

Conclusions:

The 48 hour LC50 for Daphnia magna is 1.50 mg/L.

Executive summary:

Method

The standard static acute daphnid toxicity test method was used to expose Daphnia magna to DOWFAX 3B2 surfactant. The test sample was prepared by R. C. Marriott, reference no. 1-121-1. It is an 80/20 mixture of linear C10, mono/di alkylated diphenyl oxide, sulfonated to an average degree of 2.00. The concentrations are reported as 100% active. The Daphnia were exposed for 48 hours in dechlorinated Lake Huron water at 17°C.

Results

Static Acute Daphnia magna Toxicity in Static Water - 48 Hours

1. Observed Data

No kill level <0.56 mg/ℓ

Partial kill 0.56 mg/ℓ

100% kill 5.60 mg/ℓ

2. Calculated LCX Data (95% confidence limits are in parentheses after value)

LC10 0.59 mg/ℓ (0.39-0.77)

LC50 1.50 mgℓ (1.24-1.80)

LC90 3.78mg/ℓ (2.96-5.49)

Slope 3.19 (234-4.03)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.5 mg/L

Additional information

There is a single short-term study on the toxicity of DOWFAX™ 3B2 to freshwater aquatic invertebrates.

The 48 h LC50 of DOWFAX™ 3B2 to daphnia magna was 1.5 mg/L (a.i).