Hydantoin

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Characterization of osmotic restraints on lettuce fruit germination.

Author:

Reynolds, T.

Year:

1975

Bibliographic source:

Ann. Bot. 39, 791-796 (1975a)

Reference 2

Reference Type:

publication

Title:

pH Restraints on lettuce fruit germination.

Author:

Reynolds, T.

Year:

1975

Bibliographic source:

Ann Bot. 39, 797-805 (1975b)

Reference 3

Reference Type:

publication

Title:

Comparative effects of aliphatic compounds on inhibition of lettuce fruit germination.

Author:

Reynolds, T

Year:

1977

Bibliographic source:

Ann. Bot. 41, 637-648 (1977)

Reference 4

Reference Type:

publication

Title:

Comparative effects of heterocyclic compounds on inhibition of lettuce fruit germination.

Author:

Reynolds, T.

Year:

1989

Bibliographic source:

J. Exp. Bot. 40, 391-404 (1989)

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

The germination tests were carried out according to Reynolds (1975a, 1977). The germination tests were carried out for a period of 3 d at 30°C. Twenty lettuce fruits were sown on 0.5% agar in polystyrene pots (2.8 x 3.5 cm) closed by lids floating in the water baths. The inhibitory activity of the test item producing 50% inhibition of germination was determined in five replicates.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Hydantoin

EC Number:

207-313-3

EC Name:

Hydantoin

Cas Number:

461-72-3

Molecular formula:

C3H4N2O2

IUPAC Name:

hydantoin

Test material form:

not specified

Sampling and analysis

Analytical monitoring:

no

Test substrate

Vehicle:

no

Test organisms

Species:

Lactuca sativa

Plant group:

Dicotyledonae (dicots)

Details on test organisms:

- Common name: lettuce
- Plant family: Asteraceae
- Variety: Great Lakes
- Source of seed: not reported
- Prior seed treatment/sterilization: not reported
- Historical germination of seed (germination of seed lot tested): not reported
- Seed storage: not reported

Study design

Test type:

other: germination

Study type:

laboratory study

Substrate type:

other: agar

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

30°C

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Species:

Lactuca sativa

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

67 other: mol/m3

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

germination

Remarks:

of Great Lakes

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The toxicity of Hydantoine to lettuce (Lactuca sativa cv. Great Lakes) was examined in laboratory studies using agar as growth medium. Lactuca sativa was exposed to the test item in sealed plastic containers for 3 d at 30°C and the concentration causing an 50% inhibition of germination was determined. The nominal EC50 was determined to be 67 mol/m3.