Tin antimony grey cassiterite

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

2000-03-13 to 2000-03-15

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

PART I (1984)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

(1992)

Test guideline 3

Qualifier:

according to guideline

Guideline:

other: ISO Int. Standard (1996)

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tin antimony grey cassiterite

EC Number:

269-105-9

EC Name:

Tin antimony grey cassiterite

Cas Number:

68187-54-2

Molecular formula:

Sn(2-x-y)Sb(x)V(y)O4 0,04≤x≤0,50 0≤y≤0,10

IUPAC Name:

Tin antimony cassiterite

Test material form:

solid: particulate/powder

Details on test material:

- Name of test material: Tin-Antimony-Grey
- Substance type: inorganic pigment
- Physical state: solid, grey powder
- Stability under test conditions: at least 96 hours
- Storage condition of test material: At room temperature in the dark

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
not applicable

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

no data

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: test solution was prepared at 100 mg/L applying magnetic stirring for 45 minutes followed by 1 minute of treatment with ultrasonic waves.
- Controls: blank
- Evidence of undissolved material: final test solution was agrey suspension with some undissolved particles.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Species: Daphnia magna (Crustacea, Cladocera)
- Age at study initiation: <24 hours

Study design

Test type:

static

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

218 mg CaCO3/L

Test temperature:

21.2-21.5 °C

pH:

Blank-control:
-test start: 8.0
-test end: 7.9

100mg/L:
test start: 7.9
test end: 7.9

Dissolved oxygen:

Blank-control:
- test start: 9.8 mg O2/L
- test end: 9.5 mg O2/L
Test vessel (100mg/L):
- test start: 10.1 mg O2/L
- test end: 9.6 mg O2/L

Salinity:

not applicable

Nominal and measured concentrations:

nominal test concentration: 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: containing 80 mL test solution
- No. of organisms per vessel: 10 daphnids
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- test water: ISO-medium (hardness: 218 mg CaCO3/L)

EFFECT PARAMETERS MEASURED:
- immobility of daphids at 24 and 48 hours

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Deposits were observed during the entire test period at 100 mg/L.

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: Deposits were observed during the entire test period at 100 mg/L.

Details on results:

- Biological observations: acute immobilisation of Daphnia magna was not observed at 100 mg/L after 48 hours of exposure.
- Deposits were observed during the entire test period at 100 mg/L due to the material being tested as dispersion.
Please also refer to table 1 in the field "Any other information on results incl tables" below.

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

no data

Any other information on results incl. tables

Table 1: Incidence of immobility

TIN-ANTMONY- GREY (mg/L)	Vessel number	Number Daphnia exposed	Response at 24 hours *		Response at 48 hours
			number	%	number	%
Blank-control	A	10	0	0	0	0
	B	10	0	0	0	0
100*	A	10	0(2)	0	0	0
	B	10	0	0	0	0

* Between brackets: number of daphnids observed trapped at the surface

**Deposits were observed during the entire test period in the 100 mg/L solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

TIN-ANTIMONY-GREY did not inhibit mobilisation of Daphnia magna at 100 mg/L (nominal, dispersed) during 48 hours of exposure in a limit test according to OECD TG 202. Thus, the NOEC for immobilisation of Daphnia magna is estimated with >= 100 mg/L (nominal, dispersed) and the corresponding 48-h EC50 with >100 mg/L (nominal, dispersed).