Sodium 3-[[5-[(diethylamino)sulphonyl]-2-methoxyphenyl]azo]-4-hydroxynaphthalene-1-sulphonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

water

Details on test solutions:

The stock solution of the test substance was prepared in the dilution water. 100 mg of the test substance was weighed into 1000 mL of the dilution water for the preliminary test. The stock solution was ultrasonicated for 10 minutes. The concentrations of solutions used in the preliminary test were obtained by dilution of the stock solution with dilution water.

Test organisms (species):

Daphnia magna

Details on test organisms:

The organism used in this test system is the species Daphnia magna. Daphnia magna Straus were obtained by aperiodic parthenogenesis from own laboratory breeding. Daphnia were fed by a mixture of algae. The sensitivity of the test species is periodically monitored by testing with the reference substance, potassium dichromate.
The grow-up gravid females are separated into dilution water. Young daphnia born in 24 hours are segregated to the handling container from which they entered the experiment. At handling a care should be taken of not to penetrate the air under animal’s shell.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

2.47 mmol/l Ca+Mg

Test temperature:

18-22°C

pH:

100 mg/l 48 h: 7.9
100 mg/l 0 h: 8.3

Dissolved oxygen:

The medium was oxygen saturated at the beginning of the test.

Nominal and measured concentrations:

1, 5, 10, 50 and 100 mg/l

Details on test conditions:

Temperature: 18-22 °C, with a variation of 1°C at each individual test
Exposure time: 48 hours
Lighting: 16-hours light and 8-hours dark
Number of test animals: 20 for each concentration and control
Volume of test substance solution: 50 ml (least 2 ml per individual)
Concentration of dissolved oxygen: higher than 3 mg·l -1

Reference substance (positive control):

yes

Remarks:

Potassium dichromate

Duration:

24 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

24 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

EC50 48 h > 100 mg/l

Executive summary:

No toxic effects on Daphnia was observed under the test concentrations.

Description of key information

EC50 = 100 mg/l

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

One test on Daphnia Magna have been performed, showing no mortality at the tested concentrations.

The key study has been performed for different concentration from 0 to 100 mg/l based on the active ingredient.
The value of EC50 > 100 mg/l has been established.