Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-778-1 | CAS number: 110-56-5
Genetic toxicity: in vivo
Administrative data
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study without detailed documentation
Data source
Reference
- Reference Type:
- publication
- Title:
- In Vivo Micronucleus Test of Methylcyclopentane and 1,4-Dichlorobutane
- Author:
- Kyung-Taek Rim
- Year:
- 2 011
- Bibliographic source:
- J. of the Korea Society for Environmental Analysis, 2:89-93
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Version / remarks:
- 1997
- GLP compliance:
- not specified
- Type of assay:
- other: Mouse bone marrow micronucleus test
Test material
- Test material form:
- liquid
- Details on test material:
- Provider: Sigma, St Louis MO, USA, Lot No. 1323541
Constituent 1
Test animals
- Species:
- mouse
- Strain:
- ICR
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS:
- Strain: Mouse / ICR
- Number and sex: 24 males (6 per group)
- Breeder: Japan SLC Inc., Shizuoka, Japan
- Age: 7 weeks
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- olive oil
- Duration of treatment / exposure:
- single oral administration, test was condcuted 24 h after treatment
- Frequency of treatment:
- once
- Post exposure period:
- 24 h
Doses / concentrationsopen allclose all
- Dose / conc.:
- 0 mg/kg bw/day (nominal)
- Dose / conc.:
- 500 mg/kg bw/day (nominal)
- Dose / conc.:
- 1 000 mg/kg bw/day (nominal)
- Dose / conc.:
- 2 000 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 6
- Control animals:
- yes
- Positive control(s):
- yes, Mitomycin C
Examinations
- Tissues and cell types examined:
- bone marrow cells
- Details of tissue and slide preparation:
- The femur was used for the preparation of bone marrow cells. Therefore, bone marrow was flushed from the femurs and spread onto slides. The slides were air-dried, fixed with ethanol, and stained with a fluorescent DNA specific stain.
- Statistics:
- One way Analysis of Variance (ANOVA, P<0.001) test and Dunnett's Method (P<0.05) using SigmaStat v. 3.11.
Results and discussion
Test results
- Key result
- Sex:
- male
- Genotoxicity:
- negative
- Toxicity:
- no effects
- Negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
- Conclusions:
- Under the conditions tested, 1,4-dichlorobutane does not have mutagenic potential on bone marrow cells of mice.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
