Isobutyrophenone

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Isobutyrophenone

EC Number:

210-275-0

EC Name:

Isobutyrophenone

Cas Number:

611-70-1

Molecular formula:

C10H12O

IUPAC Name:

2-methyl-1-phenylpropan-1-one

Details on test material:

- Name of test material (as cited in study report): Isobutyrophenone (TK 40392)
- Physical state: Liquid / colorless, clear
- Analytical purity: 99.2 area-%
- Batch No.: 090727T102
- Storage condition of test material: the test substance was stored at ambient temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test solutions were prepared separately for each test concentration. The stock solution vessels and test vessels were saturated with test solution for approx 1 day before the start of exposure. The test substance was homogenized by shaking the container before use. For each concentration, the test substance was weighed into a 1 L volumetric flask and filled to the mark with test medium. The solution was then be stirred for approximately 10 min at 20 ± 2 °C. The solutions were checked visually to ensure that the test substance is completely dissolved.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flea
- Source: Originally from "Institut National de Recherche Chimique appliquee, France", and has been bred from 1978 in the Laboratories of BASF
- Age at study initiation: < 24 hours (at least 3rd brood progeny)
- Feeding during test: no

CULTURE CONDITIONS
- Photoperiod: 16 hours light, 8 hours dark
- Culture media: synthetic fresh water (Elendt M4) prepared according to OECD 202
- Temperature: 20 ± 1 °C
- Diet: unicellular algae Desmodesmus subspicatus
- Feeding: Daily
- Renewal of the culture water: Twice weekly

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

2.48 mmol/L

Test temperature:

19.5 – 20.1 °C

pH:

7.9 -8.1

Dissolved oxygen:

8.9 -9.1 mg/L

Nominal and measured concentrations:

Nominal test concentrations: control, 4.6, 10.0, 22.0, 46.0 and 100 mg/L
Mean measured test concentrations: -, -, -, 12.4, 24.0, 48.0 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, nominal volume, fill volume: glass beakers, 50 ml, 50 ml
- Aeration: none
- Renewal rate of test solution: 24 h
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A synthetic fresh water (Elendt M4) is used as media for culture and test purposes.
- Alkalinity: 0.90 mmol/L
- Ca/mg ratio: about 4 : 1
- Conductivity: 584 μS/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: pH and oxygen content of the test solutions was measured after 0 h and 24 h (new test solutions) and after 24 h and 48 h (old test solutions) in replicate 1 of each tested concentration. Temperature was measured continuously.

OTHER TEST CONDITIONS
- Photoperiod: 16 hours light : 8 hours darkness
- Light intensity: about 172 – 654 lux at a wave length of 400 -750 nm

EFFECT PARAMETERS MEASURED: immobilization and abnormal behaviour and appearance after 0, 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study: yes
- Results used to determine the conditions for the definitive study: the 48 h EC50 was between 10 and 100 mg/L

Reference substance (positive control):

yes

Remarks:

potassium dichromate (K2Cr2O7)

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC0

Effect conc.:

>= 48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

EC100

Effect conc.:

> 48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: none

Results with reference substance (positive control):

The EC50 (24h) of the reference substance potassium dichromate was 1.0 mg/L. Date of the last control experiment: 15 Mar 2010