4-chlorotoluene

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study according OECD 474

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)

Principles of method if other than guideline:

The micronucleus test was employed to investigate P-Chlorotoluene in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The known clastogen and cytostatic agent, cylophosphamide,served as positive control.
The treated animals received a single intraperitoneal administration of either p-chlorotoluene or cyclophosphamide. The femoral marrow of groups treated with p-chlorotoluene was prepared 16, 24 and 48 hours after administration. All negative and positive control animals were sacrificed
after 24 hours. The doses of p-chlorotoluene and the positive control, cyclophosphamide, were 1000 and 20 mg/kg body weight, respectively.

GLP compliance:

yes

Type of assay:

micronucleus assay

Test material

Test material information

Constituent 1

Reference substance name:

4-chlorotoluene

EC Number:

203-397-0

EC Name:

4-chlorotoluene

Cas Number:

106-43-4

Molecular formula:

C7H7Cl

IUPAC Name:

4-chlorotoluene

Details on test material:

p-chlorotoluene - content: 99.81 %

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Administration / exposure

Route of administration:

intraperitoneal

Duration of treatment / exposure:

single administration

Frequency of treatment:

single administration

Post exposure period:

16, 24, or 48 hours after administration

Doses / concentrations

Remarks:

Doses / Concentrations:
1000 mg/kg bw disssolved in corn oil, dosage based on a pilot test
Basis:
nominal conc.

No. of animals per sex per dose:

5 males and 5 females per group

Control animals:

yes

Examinations

Tissues and cell types examined:

bone-marrow erythroblasts

Results and discussion

Test results

Sex:

male/female

Genotoxicity:

negative

Toxicity:

yes

Vehicle controls validity:

valid

Negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

general toxicity:
compound-related signs of toxicity observable until sa-
crifice 16, 24 and 48 hours after the administration:
apathy, roughened fur, staggering gait, spasms, twitch-
ing, shivering and difficulty in breathing; 
death of 2/40 treated animals during the test period; autopsy findings:
slightly inflated lungs, spotted livers

no indications of a clastogenic effect of p-chlorotoluene were found; the ratio of polychromatic to normochromatic erythrocytes was not altered.
The positive contol was functional.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative

Executive summary:

method: the micronucleus test was employed to investigate p-chlorotoluene in male and female mice for a possible clastogenic effect on the chromosomes of bone-marrow erythroblasts. The treated animals received a single intraperitoneal administration of either p-chlorotoluene or cyclophosphamide. The femoral marrow of groups treated with p-chlorotoluene was prepared 16, 24 and 48 hours after administration. All negative and positive control animals were sacrificed after 24 hours. The doses of p-chlorotoluene and the positive control, cyclophosphamide, were 1000 and 20 mg/kg body weight, respectively.

result: negative; no indications of a clastogenic effect of p-chlorotoluene were found after a single intraperitoneal treatment with 1000 mg/kg.