Ethyl 2-naphthyl ether

Genetic toxicity: in vivo

Administrative data

Endpoint:

genetic toxicity in vivo, other

Remarks:

Drosophila SLRL assay

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from peer reviwed publication

Data source

Reference

Reference Type:

publication

Title:

Study of artificial flavouring substances for mutagenicity in the Salmonella/microsome, basic and micronucleus tests.

Author:

Wild, D.

Year:

1983

Bibliographic source:

Food and Chemical

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: Sex linked recessive lethal mutation assay (Wuergler et al., 1977)

Principles of method if other than guideline:

Drosophila SLRL test test was performed to determine the mutagenic nature of 2-Ethoxynaphthalene

GLP compliance:

not specified

Type of assay:

Drosophila SLRL assay

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-naphthyl ether

EC Number:

202-226-7

EC Name:

Ethyl 2-naphthyl ether

Cas Number:

93-18-5

Molecular formula:

C12H12O

IUPAC Name:

2-ethoxynaphthalene

Specific details on test material used for the study:

- Name of test material: 2-Ethoxynaphthalene
- Molecular formula: C12H12O
- Molecular weight: 172.226 g/mol
- Subsatnce type: Organic
- Physical state: Solid
- Purity: No data

Test animals

Species:

Drosophila melanogaster

Strain:

not specified

Details on species / strain selection:

No data

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: No data
- Age at study initiation: Mature sperm
- Weight at study initiation: No data
- Assigned to test groups randomly: [no/yes, under following basis: ] No data
- Fasting period before study: No data
- Housing: No data
- Diet (e.g. ad libitum): No data
- Water (e.g. ad libitum): No data
- Acclimation period: No data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): No data
- Humidity (%): No data
- Air changes (per hr): No data
- Photoperiod (hrs dark / hrs light): No data

IN-LIFE DATES: From: To: No data

Administration / exposure

Route of administration:

oral: feed

Vehicle:

- Vehicle(s)/solvent(s) used: Solutions or emulsions of test substances to be fed to the flies were prepared in 5% saccharose, with the addition of 2% ethanol and 2% Tween 80 for compounds that were poorly soluble in water.
- Justification for choice of solvent/vehicle: No data
- Concentration of test material in vehicle: 25mM
- Amount of vehicle (if gavage or dermal): No data
- Type and concentration of dispersant aid (if powder): No data
- Lot/batch no. (if required): No data
- Purity: No data

Details on exposure:

No data

Duration of treatment / exposure:

No data

Frequency of treatment:

No data

Post exposure period:

No data

Doses / concentrations

Dose / conc.:

25 other: mM

No. of animals per sex per dose:

No data

Control animals:

not specified

Positive control(s):

No data

Examinations

Tissues and cell types examined:

Sex linked recessive lethal chromosomes

Details of tissue and slide preparation:

No data

Evaluation criteria:

Increase in the number of recessive lethal chromosomes

Statistics:

No data

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

not specified

Negative controls validity:

not specified

Positive controls validity:

not specified

Remarks on result:

other: No mutagenic effect were observed.

Additional information on results:

No data

Any other information on results incl. tables

Table: Results of Basc tests on Drosophila for sex-linked recessi',e lethal mutations

Concentration(mM)	No.of sex-linked recessive lethal chromosomes tested in
	Brood I		Brood II		Brood III
25	8/2383	0.34	3/2379	0.13	5/2423	0.21

Applicant's summary and conclusion

Conclusions:

2-Ethoxynaphthalene gave negative gene mutation results in the Drosophila SLRL test performed using male Drosophila melanogaster species.

Executive summary:

Gene mutation toxicity study was performed to determine the mutagenic nature of 2-Ethoxynaphthalene using Drosophila SLRL test in vivo. Solutions or emulsions of test substances to be fed to the flies were prepared in 5% saccharose, with the addition of 2% ethanol and 2% Tween 80 for compounds that were poorly soluble in water. 2-Ethoxynaphthalene was used at dose levels of 25mM. Sex linked recessive lethal mutation were noted in the chromosomes. 2-Ethoxynaphthalene gave negative gene mutation results in the Drosophila SLRL test performed using male Drosophila melanogaster species.