5-methyl-2-(1-methylvinyl)cyclohexan-1-ol

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study with chemical control analysis

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Isopulegol

EC Number:

201-940-6

EC Name:

Isopulegol

Cas Number:

89-79-2

Molecular formula:

C10H18O

IUPAC Name:

(1R,2S,5R)-2-Isopropenyl-5-methylcylohexanol

Details on test material:

- Name of test material (as cited in study report):L-Isopulegol
- Substance type: chemical
- Physical state: liquid
- Analytical purity: 99.89 %
- Lot/batch No.: Muster 7 CH
- Storage condition of test material: stored at ambient temperature and under nitrogen
- Water solubility: 3 g/L at 21.9 °C (pH 5.04)

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: At the start of the exposure (0 h) samples from vessels without daphnids and at the end
of the exposure (48 h) samples from vessels with daphnids were analyzed.
- Sample storage conditions before analysis: ambient temperature

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
The test substance was homogenized by shaking the container before use. Each test solution was prepared separately (differential loading) by directly adding test substance to test medium. For each concentration, the test substance was pipetted into a 1 L volumetric flask and filled to the mark with test medium. The solution was stirred for approximately 10 min at 20 ± 2 °C. The solutions were checked visually to ensure that the test substance is completely dissolved.
The volume of the test substance required for preparation of each nominal concentration was calculated from the density.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea
- Source: Institut National de Recherche Chimique Appliquée, France in 1978
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20.1 - 20.5 °C

pH:

7.8 - 8.0

Dissolved oxygen:

8.3 - 8.8

Nominal and measured concentrations:

Nominal: 0, 4.6, 10, 22, 46, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glasss flask (nominal volume 50 ml) with glass stopper
- Type (delete if not applicable): closed
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 0.1 animals/ml

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: M4-medium
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 h day night rhythm
- Light intensity: about 20 - 850 lux at a wave length of 400 - 750 nm

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility after 0, 24, 48 h

TEST CONCENTRATIONS
- Spacing factor for test concentrations:2.2

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

53.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility