Reaction mass of Chromate(2-), [3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)] [N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthalenyl]]-, lithium sodium and Chromate(2-), [3-hydroxy-4-[(2-hydroxy-1-naphthalenyl)azo]-7-nitro-1-naphthalenesulfonato(3-)][N-[7-hydroxy-8-[(2-hydroxy-5-nitrophenyl)azo]-1-naphthalenyl]acetamidato(2-)]-, lithium sodium

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1984-12-18 to 1984-12-22

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Weighed quantities of the test item plus 10 mL of deionised water were added to 15 L bioassay water in each of 11 labelled 19 L glass jars to produce 15 L of each of the nominal concentrations: 0.2, 0.3, 0.44 0.67, 0.99, 1.5, 2.3, 3.4, 5.1, 7.6, 11.4 ppm.

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: rainbow trout
- Source: Mr Lassen Trout Farm, Red Bluff, California, USA
- Weight at study initiation (mean and range, SD): range 0.76 - 1.01 g

ACCLIMATION
- Acclimation period: 5 d
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: Ralston Purina Trout Chow ad lib.

FEEDING DURING TEST
- Amount: no feeding during test

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Hardness:

159 - 162 mg/L CaCO3

Test temperature:

13.1 - 15.0°C

pH:

7.00 - 7.66

Dissolved oxygen:

6.2 - 9.8 mg/L

Nominal and measured concentrations:

Nominal: 0 (control), 0.2, 0.3, 0.44 0.67, 0.99, 1.5, 2.3, 3.4, 5.1, 7.6, 11.4 ppm

Details on test conditions:

TEST SYSTEM
- Test vessel: glass jar
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Johnson County Rural Water District No. 2
- Alkalinity: 55 - 61 mg/L CaCO3
- Culture medium different from test medium: no
- Intervals of water quality measurement: daily

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16:8 light:dark

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): mortality, sublethal effects

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

0.84 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 95% CI: 0.78 - 0.91 mg/L

Validity criteria fulfilled:

not specified

Conclusions:

The 96-h LC50 to fish was determined at 0.84 mg/L (Oncorhynchus mykiss).

Description of key information

The 96-h LC50 to fish (Pimephales promelas) in the key study was determined at 2.0 mg/L.

As results with warmwater fish which are used to constant environmental conditions are more reliable than tests with coldwater species, a 96 -h LC50 for rainbow trout at 0.84 mg/L was used as supporting study. Coldwater species are known to significantly vary in their toxicity response depending on the environmental conditions, especially with changing temperature (annual/seasonal life cycle). Warmwater species react more independently as their physical fitness is not related to the time of the year when testing takes place.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2 mg/L