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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
Only basic data given, no GLP and guideline study, the test substance was tested in a different composition as compared to the composition of the registered substance.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1978
Report date:
1978

Materials and methods

Principles of method if other than guideline:
The test substance is administered intraperitoneally in graduaded doses to several groups of mice. Subsequently observations of effects and deaths are made.
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[2-[4-(dimethylamino)phenyl]vinyl]-1,3,3-trimethyl-3H-indolium acetate
EC Number:
275-654-5
EC Name:
2-[2-[4-(dimethylamino)phenyl]vinyl]-1,3,3-trimethyl-3H-indolium acetate
Cas Number:
71598-17-9
Molecular formula:
C21H25N2.C2H3O2
IUPAC Name:
2-{2-[4-(dimethylamino)phenyl]vinyl}-1,3,3-trimethyl-3H-indolium acetate

Test animals

Species:
mouse
Strain:
NMRI
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: male: 27.5 g, female: 26 g
- Diet: Herilan MRH-Haltung, Alleinfutter für die Haltung von Mäusen, Ratten und Hamstern, Heinrich EGGERSMANN KG, Rinteln

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
CMC (carboxymethyl cellulose)
Details on exposure:
Application volume: 10 mL/kg
Form of administration: 0.464 - 31.6 % dispersion in distilled water

VEHICLE
- Amount(s) applied: 0.5 % CMC in distilled water
Doses:
46.4, 68.1, 100, 147 mg/kg bw
No. of animals per sex per dose:
5 males and 5 females
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels
Key result
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 80 mg/kg bw
Based on:
test mat.
Mortality:
46.4 mg/kg bw: no animals died
68.1 mg/kg bw: 3 animals died
100 mg/kg bw: 8 animals died
147 mg/kg bw: 10 animals died
Clinical signs:
Death and poor general state on the 1st day of the study. At the beginning, loss of weight in some animals; nonspecific signs.
Gross pathology:
Adipose tissue: Slightly colored
Sacrified animals: No intraabdominal test substance precipitates or adhesions.

Applicant's summary and conclusion