β-methylaziridine-1-propiononitrile

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1980-04-15

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: well-documented, scientifically acceptable study report

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Principles of method if other than guideline:

according to BASF-internal standard

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

β-methylaziridine-1-propiononitrile

EC Number:

223-798-4

EC Name:

β-methylaziridine-1-propiononitrile

Cas Number:

4078-19-7

Molecular formula:

C6H10N2

IUPAC Name:

3-(aziridin-1-yl)butanenitrile

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

The animals were offered a standard animal laboratory diet. Fasting period 15 - 20 h before administration.

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

olive oil

Details on oral exposure:

Application form: emulsion
Concentrations used: 1.36, 2, 2.94, 4.3 and 5.22 % (G/V)
Application volume: 5 mL/kg bw

Doses:

68.1, 100, 147, 215 and 261 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

111 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Slope factor 1.38

Effect levels 2

Sex:

male

Dose descriptor:

LD50

Effect level:

121 mg/kg bw

Based on:

test mat.

Remarks on result:

other: Slope factor 1.36

Effect levels 3

Sex:

female

Dose descriptor:

LD50

Effect level:

ca. 110 mg/kg bw

Based on:

test mat.

Remarks on result:

other: derived by interpolation

Mortality:

Male animals: 68.1 mg/kg bw: no deaths after 14 days; 100 mg/kg bw: 2/5 after 7 days; 147 mg/kg bw: 3/5 after 7 days; 215 mg/kg bw: 5/5 after 7 days; 261 mg/kg bw: 1/5 after 1 day, 4/5 after 7 days, 5/5 after 14 days
Female animals: 68.1 and 100 mg/kg bw: 1/5 after 14 days; 147 mg/kg bw: 3/5 after 7 days, 5/5 after 14 days; 215 mg/kg bw: 1/5 after 2 days, 5/5 after 7 days; 261 mg/kg bw: 5/5 after 7 days

Clinical signs:

other: Dyspnea, gasping, apathy, abnormal position, staggering, atony, trembling, spastic gait, ruffled fur, alopecia, exsiccosis, poor general state

Gross pathology:

Animals that died: heart: acute dilatation (right); acute congestive hyperemia; kidneys: papillae yellow-grey (papilla necrosis); urinary bladder: isolated bloody content
Sacrificed animals: white strips in the papilla, isolated papilla yellow (papilla necrosis)

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category III

Remarks:

Migrated information

Conclusions:

The acute oral LD50 of the test substance in olive oil was determined to be 111 mg/kg bw in male and female rats.

Executive summary:

The test substance was administered orally by gavage to male and female rats in olive oil. Doses of 68.1, 100, 147, 215 and 261 mg/kg bw were applied. Death and several clinical signs were observed. The acute oral LD50 was determined to be 111 mg/kg bw in male and female rats.