2-amino-4,6-dinitrophenol

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

4 (not assignable)

Data source

Reference

Reference Type:

secondary source

Title:

OPINION ON Picramic acid and sodium picramate COLIPA n° B28

Author:

Scientific Committee on Consumer Safety

Year:

2012

Bibliographic source:

Scientific Committee on Consumer Safety, (SCCS) 18 September 2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other:

Principles of method if other than guideline:

To assess the toxic effect of Picramic acid in rabbit by dermal application.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-4,6-dinitrophenol

EC Number:

202-544-6

EC Name:

2-amino-4,6-dinitrophenol

Cas Number:

96-91-3

Molecular formula:

C6H5N3O5

IUPAC Name:

2-amino-4,6-dinitrophenol

Test material form:

other: dark red needles

Details on test material:

- Name of test material (as cited in study report): picramic acid
- Molecular formula (if other than submission substance): C6H5N3O5
- Molecular weight (if other than submission substance): 199.12 g/mol
- Substance type: Organic
- Physical state: solid

Test animals

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

No data available.

Test system

Type of coverage:

occlusive

Preparation of test site:

other: intact and abraded

Vehicle:

other: Distilled water containing 0.05% sodium sulfite,

Controls:

not specified

Amount / concentration applied:

2.5% aqueous solution

Duration of treatment / exposure:

24 hr

Observation period:

72 hr

Number of animals:

3

Details on study design:

TEST SITE
- Area of exposure: 0.5 ml of 2.5% aqueous solution of the test material was applied on 2.5 cm² under an occlusive patch to intact and abraded skin on dorsal aspect for 24 hr.
- % coverage: No data available.
- Type of wrap if used: No data available.


REMOVAL OF TEST SUBSTANCE: No data available.

SCORING SYSTEM:

Results and discussion

In vivo

Results

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: 72 hour

Reversibility:

no data

Remarks on result:

other: No reaction was observed

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

Under the condition of the test, a 2.5% aqueous solution of Picramic acid was not irritant to rabbit skin.

Executive summary:

In acute dermal irritation test of Picramic acid was determined in rabbit skin for over 72 hour. 0.5 ml of 2.5% aqueous solution of the test material was applied on 2.5 cm² under an occlusive patch to intact and abraded skin on dorsal aspect for 24 hr. No reaction was observed over 72 hr. Therefore Picramic acid was found to be not irritant to rabbit skin.