7-anilino-4-hydroxynaphthalene-2-sulphonic acid

Toxicity to aquatic algae and cyanobacteria

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

calculation (if not (Q)SAR)

Remarks:

Migrated phrase: estimated by calculation

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from computational model developed by USEPA

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Modelling Database - ECOSAR v.1.11

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

other: green algae

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Remarks on exposure duration:

Estimated data

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

708.246 mg/L

Nominal / measured:

estimated

Conc. based on:

test mat.

Basis for effect:

growth rate

Remarks on result:

other: Neutral Organic SAR (Baseline Toxicity)

Validity criteria fulfilled:

not specified

Conclusions:

The estimated EC50 values for aquatic algae after 96 hours was 708.246 mg/l on the basis of growth rate.

Executive summary:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours EC50 was estimated to be 708.246 mg/l on green algae for substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid with growth inhibition effects.Thus, based on the EC50 value, test substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Description of key information

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10, the 96 hours EC50 was estimated to be 708.246 mg/l on green algae for substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid with growth inhibition effects.Thus, based on the EC50 value, test substance can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.

Key value for chemical safety assessment

EC50 for freshwater algae:

708.246 mg/L

Additional information

Predicted data for the target chemical4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid (CAS No. 119-40-4) and the study for its read across substance were reviewed to summarize the following information:

Based on the QSAR prediction done using the EPI Suite ECOSAR version 1.10 (EPI Suite, 2016), the 96 hours EC50 was estimated to be 708.246 mg/l on green algae for substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid with growth inhibition effects.

The effect of test item disodium 4-amino-5-hydroxynaphthalene-1,7-disulphonic acid, CAS No. 130-23-4 was studied [UERL Study Report, Sustainability Support Services (Europe) AB (Report no. 130-23-4/01/2016/AT), 2016] on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga, growth inhibition test. The test concentration chosen for the study were 6.25mg/l, 12.5mg/l, 25mg/l, 50mg/l, 100mg/l and 200mg/l. The test concentrations were prepared using stock solution of the test item using mineral media. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of the test item. EC50 calculated graphically through probit analysis was observed to be >200mg/l.

 

Freshwater algal growth inhibition test was carried out on Desmodesmus subspicatus with the substance 4-Amino-5-hydroxynaphthalene-1,7 -disulp

honic acid according to OECD Guideline 201 [ABITEC study report, Sustainability Support Services (Europe) AB (study report no. 506/16, 2016]. The stock solution (200 mg/l) was prepared by dissolving light grey powder in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture. Conc. of test chemical used for the study was 2.2, 11, 25, 55 and 120 mg/L, respectively. The test was performed under static conditions in a static fresh water system at a temp. of 23±2°C. Initial cell density of test organism used was 5x10(3) cells/ml. Determination of cell counting involve the use electronic particle counter. ErC50 was calculated using non-linear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance 4-Amino-5-hydroxynaphthalene-1,7-disulphonic acid in Desmodesmus subspicatus was determined to be 129.4 mg/L on the basis of effects on growth rate in a 72 hour study.

 

Based on the overall reported results for target and its read across substance, it can be concluded that the substance 4-Hydroxy-7-(phenylamino) naphthalene-2-sulfonic acid can be considered as non-toxic to aquatic organisms and thus cannot be classified as hazardous as per the CLP criteria.