1,3-Cyclopentanedione, 2-[2-(3,4-dihydro-6-methoxy-1(2H)-naphthalenylidene)ethyl]-2-methyl-

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Well documented study according to international accepted guidelines and GLP compliant.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

2-[(2E)-2-(6-Methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethyl]-2-methylcyclopentane-1,3-dione

EC Number:

618-321-5

Cas Number:

899-79-6

Molecular formula:

C19H22O3

IUPAC Name:

2-[(2E)-2-(6-Methoxy-3,4-dihydronaphthalen-1(2H)-ylidene)ethyl]-2-methylcyclopentane-1,3-dione

Test material form:

solid: crystalline

Details on test material:

Test item: Methyl-secodione
CAS number: 899-79-6
Molecular formula: C19H22O3
Molecular weight: 298.00 g/mol
Batch number L4B021N
Physical state: Solid, crystalline substance
Colour: Beige
Storage: At room temperature, dry place

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, non-adapted

Details on inoculum:

Type of inoculum: aerobic mixed bacterial culture
Source: Municipal wastewater treatment plant, Esztergom-Kertváros

Duration of test (contact time):

28 d

Details on study design:

Preparation of inoculum test culture:
Before preparation of inoculum test culture, the dry substance content of the stock culture was determined. The measured value was 1.8 g/L. 250.0 ml inoculum test culture with 1.0 g/L dry substance was made by mixing 137 ml of stock culture with 113 ml mineral medium.
Preparation of reference solution:
For the reference solution 320.22 mg sodium acetate was diluted in 100 ml mineral medium water.
Preparation of test vessels:
Following test vessels were prepared and analysed for the study:
- Test item (in two replicates)
- Blank control (in two replicates)
- Reference solution (positive control)
- Reference and test item solution (toxicity control)
- Sterilized solution of test item (abiotic control)

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (O2 consumption)

Value:

28

Sampling time:

28 d

Details on results:

The measured BOD values for test item, positive and toxicity controls were corrected with the results of blank control.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

In this biodegradability of "Methyl-secodione" (CAS No. 899-79-6) the results indicated that the test item is not ready biodegradable in the aquatic environment.