Benzenepropanoic acid, 3,5-bis(1,1-dimethylethyl)-4-hydroxy-, isotridecyl ester

Endpoint summary

Description of key information

Acute oral toxicity:
rat (Sprague-Dawley) m/f; LD50 > 5000 mg/kg bw [EU Method B.1, GLP; test item: structural analogue with EC 413-750-2]
Acute inhalation toxicity:
- rat (Sprague-Dawley) m/f; LC50 > 7.5 mg/L air (4h) [OECD TG 403, GLP; test item: structural analogue with EC 413-750-2]
Acute dermal toxicity:
rat (Sprague-Dawley) m/f; LD50 > 2000 mg/kg bw (24h) [EU Method B.3; test item: structural analogue with EC 413-750-2]

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study obtained through inquiry process; SNIF file obtained from ECHA.

Qualifier:

according to guideline

Guideline:

EU Method B.1 (Acute Toxicity (Oral))

Deviations:

not specified

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Species:

rat

Sex:

male/female

Route of administration:

oral: unspecified

Vehicle:

other: Methylcellulose 400 cps water solution

Doses:

5000 mg/kg bw

No. of animals per sex per dose:

- Male: 5
- Female: 5

Control animals:

not specified

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

not specified

Mortality:

- Male: 5000 mg/kg bw; Number of animals: 5; Number of deaths: 0
- Female: 5000 mg/kg bw; Number of animals: 5; Number of deaths: 0

Clinical signs:

other: Signs of toxicity related to dose levels: Piloerection was observed only two hours after test article administration in two male and three female rats.

Gross pathology:

Effects on organs: At the gross pathology examination carried out at the end of the observation period no changes were noted in all necropsied
animals.

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: EU-GHS

Conclusions:

LD50 (rat) > 5000 mg/kg bw
The test substance, when administered by oral route to rat, under the conditions adopted in this study, did not cause overt signs of toxicity.

Executive summary:

The acute oral toxicity of the structural analogue has been determined in a GLP test with rats according to EU method B.1 in a limit test. The LD50 value was > 5000 mg/kg bw, the test substance showed no toxicity under the conditions of the test.

Since EC 700-397-7 is a near analogue to the test substance (EC 413-750-2), the experimental data from this substance were used in a read-across approach.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

The data has been extracted from ECHA databases. The files were first migrated from the SNIF (Structured Notification Interchange Format) format (used under Directive 67/548/EEC) into the IUCLID 5 format (used under the REACH Regulation) and represent all of the information that ECHA currently holds on this endpoint, which were submitted in the framework of a notification at least 12 years previously (as per Article 25(3)) for the substance with the same EC number for which you inquired.

Acute toxicity: via inhalation route

Link to relevant study records

Reference

Endpoint:

acute toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study obtained through inquiry process; SNIF file obtained from ECHA.

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

not specified

GLP compliance:

yes

Test type:

other: no data

Limit test:

yes

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Route of administration:

inhalation

Type of inhalation exposure:

nose/head only

Vehicle:

not specified

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

4 h

Concentrations:

The nominal concentrations administered were 87.85 mg/L (group 1) and 70.14mg/L (group 2).

No. of animals per sex per dose:

- Male: 5
- Female: 5

Control animals:

not specified

Sex:

male/female

Dose descriptor:

LC50

Effect level:

> 7.5 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

- Male: 4.07 mg/L; Number of animals: 5; Number of deaths: 0
- Male: 7.53 mg/L; Number of animals: 5; Number of deaths: 0
- Female: 4.07 mg/L; Number of animals: 5; Number of deaths: 0
- Female: 7.53 mg/L; Number of animals: 5; Number of deaths: 0

Clinical signs:

other: Signs of toxicity related to dose levels: An unkempt appearance was noted for all Group 1 animals immediately post dose and one animal of Group 2 appeared subdued and showed hunched posture over the first 24h post dose. Otherwise no other abnormalities w

Body weight:

There was no effect on body weight profile following exposure to the test substance.

Gross pathology:

Necropsy examination:
All Group 1 animals were seen to have no abnormalities.
Slightly mottled lungs in all but 2 Group 2 animals and pale/discoloured lungs were noted in one Group 2 male and 1 Group 2 female. These were
deemed to be in accordance with normal background findings in acute rat studies at IRI and not attributable to treatment.

Body weight ratio:
Lung:body weight ratios were generally within normal limits for all animals, although Group 2 males tended towards the bottom of the expected
range.

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: Eu GHS

Conclusions:

LC50 (rat) > 7.5 mg/L air (4h)
The test substance did not produce any evidence of toxicity in Sprague-Dawley rats following exposure for 4 h to an atmosphere containing 7.53 mg/L air (measured gravimetrically).

Executive summary:

The acute inhalation toxicity of the structural analogue has been determined in a GLP test with rats according to OECD Guideline No. 403. The test substance did not produce any evidence of toxicity in Sprague-Dawley rats following exposure for 4 h to an atmosphere containing 7.53 mg/L air (measured gravimetrically), so the LC50 value is > 7.5 mg/L air (4h).

Since EC 700-397-7 is a near analogue to the test substance (EC 413-750-2), the experimental data from this substance were used in a read-across approach.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

The data has been extracted from ECHA databases. The files were first migrated from the SNIF (Structured Notification Interchange Format) format (used under Directive 67/548/EEC) into the IUCLID 5 format (used under the REACH Regulation) and represent all of the information that ECHA currently holds on this endpoint, which were submitted in the framework of a notification at least 12 years previously (as per Article 25(3)) for the substance with the same EC number for which you inquired.

Acute toxicity: via dermal route

Link to relevant study records

Reference

Endpoint:

acute toxicity: dermal

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study obtained through inquiry process; SNIF file obtained from ECHA.

Qualifier:

according to guideline

Guideline:

EU Method B.3 (Acute Toxicity (Dermal))

Deviations:

not specified

GLP compliance:

yes

Test type:

other: no data

Limit test:

yes

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Type of coverage:

semiocclusive

Vehicle:

not specified

Duration of exposure:

24

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

- Male: 5
- Female: 5

Control animals:

not specified

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

- Male: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 0
- Female: 2000 mg/kg bw; Number of animals: 5; Number of deaths: 0

Clinical signs:

other: Signs of toxicity related to dose levels: the rats did not show any clinical sign or change in behaviour.

Gross pathology:

Effects on organs: no changes were observed in the animals killed at the end of the study.

Other findings:

Signs of toxicity (local): no reaction was observed at the application site.

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: other: EU GHS

Conclusions:

LD50 (rat) > 2000 mg/kg bw
The test substance, when administered by dermal route to rat, under the conditions adopted in this study, did not cause mortality nor toxic effects.

Executive summary:

The acute dermal toxicity of the structural analogue has been determined in a GLP test with rats according to EU methog B.3 in a limit test. The LD50 (24h) value was > 2000 mg/kg bw, the test substance did not cause mortality nor toxic effects under the conditions of the test.

Since EC 700-397-7 is a near analogue to the test substance (EC 413-750-2), the experimental data from this substance were used in a read-across approach.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Quality of whole database:

The data has been extracted from ECHA databases. The files were first migrated from the SNIF (Structured Notification Interchange Format) format (used under Directive 67/548/EEC) into the IUCLID 5 format (used under the REACH Regulation) and represent all of the information that ECHA currently holds on this endpoint, which were submitted in the framework of a notification at least 12 years previously (as per Article 25(3)) for the substance with the same EC number for which you inquired.

Additional information

Since EC 700-397-7 is a near analogue to the test substance (EC 413-750-2), the experimental data from this substance were used in a read-across approach:

Acute oral toxicity

The acute oral toxicity of the structural analogue has been determined in a GLP test with rats according to EU methog B.1 in a limit test. The LD50 value was > 5000 mg/kg bw, the test substance showed no toxicity under the conditions of the test.

Acute inhalation toxicity

The acute inhalation toxicity of the structural analogue has been determined in a GLP test with rats according to OECD Guideline No. 403. The test substance did not produce any evidence of toxicity in Sprague-Dawley rats following exposure for 4 h to an atmosphere containing 7.53 mg/L air (measured gravimetrically), so the LC50 value is > 7.5 mg/L air (4h).

Acute dermal toxicity

The acute dermal toxicity of the structural analogue has been determined in a GLP test with rats according to EU methog B.3 in a limit test. The LD50 (24h) value was > 2000 mg/kg bw, the test substance did not cause mortality nor toxic effects under the conditions of the test.

Justification for selection of acute toxicity – oral endpoint
GLP and guideline study

Justification for selection of acute toxicity – inhalation endpoint
GLP and guideline study

Justification for selection of acute toxicity – dermal endpoint
GLP and guideline study

Justification for classification or non-classification

Acute oral toxicity

The test material does not meet the criteria for classification and will not require labelling for oral toxicity in accordance with European Regulation (EC) No. 1272/2008.

Acute inhalation toxicity

The test material does not meet the criteria for classification and will not require labelling for inhalation toxicity in accordance with European Regulation (EC) No. 1272/2008.

Acute dermal toxicity

The test material does not meet the criteria for classification and will not require labelling for dermal toxicity in accordance with European Regulation (EC) No. 1272/2008.