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Diss Factsheets

Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1956
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Test procedure not according to a defined method, meets generally acceptable scientific principles but insufficient to classify according to current regulatory criteria.
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1956
Reference Type:
publication
Title:
Toxicological studies of certain alkylated benzenes.
Author:
Wolf, M.A.; Rowe, V.K.; McCollister, D.D.; Hollingworth, R.L.; Oyen, F.
Year:
1956
Bibliographic source:
AMA Arch. Ind. Health. 14:387-398.

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
No guideline available
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Ethylbenzene
EC Number:
202-849-4
EC Name:
Ethylbenzene
Cas Number:
100-41-4
Molecular formula:
C8H10
IUPAC Name:
ethylbenzene
Details on test material:
- Name of test material (as cited in study report): Ethyl benzene
- Physical state: liquid
- Analytical purity: 98%+
-Specific gravity at 20ºC: 0.86

Test animals / tissue source

Species:
rabbit
Strain:
not specified
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Source: heterogenous stock raised in the laboratory
- Diet (e.g. ad libitum): Purina rabbit chow supplemented by alfalfa hay


ENVIRONMENTAL CONDITIONS: not specified in the report

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not specified
Amount / concentration applied:
2 drops of undiluted substance were applied to the eyes
Duration of treatment / exposure:
not specified in the report
Observation period (in vivo):
Study of possible irritation was observed after 3 min, 1 hour and 1, 2 and 7 days. A 5% fluorescein stain was used to assess corneal damage after 3 mins exposure.
Number of animals or in vitro replicates:
not specified in the report
Details on study design:
not specified in the report

Results and discussion

In vivo

Results
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 7 days
Score:
0
Max. score:
0
Reversibility:
not specified
Irritant / corrosive response data:
Slight irritation to the conjunctivae (perceptible irritation), but no changes to the cornea were diagnosed.
Other effects:
not specified in the report

Any other information on results incl. tables

Slight irritation to the conjunctivae (perceptible irritation), but no changes to the cornea were diagnosed. No further data was available.

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
Ethyl benzene caused slight conjunctival irritation and no corneal injury. The results of this study suggest that ethyl benzne is slightly irritating to the eye.
Executive summary:

Ethylbenzene was evaluated for eye irritation in rabbits and under the conditions of the study caused conjunctival irritation and no corneal injury.

The results of this study suggest that ethyl benzne is slightly irritating to the eye.