Reaction mass of 4,4'-methylenediphenyl diisocyanate and o-(p-isocyanatobenzyl)phenyl isocyanate / methylene diphenyl diisocyanate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: According to guideline (84/449/EEC) with GLP

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1990

Report date:

1990

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: 84/449/EEC (Gazette of the European Community, No. L 251, of 19 Sept, 1984, page 96)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Methylenediphenyl diisocyanate

EC Number:

247-714-0

EC Name:

Methylenediphenyl diisocyanate

Cas Number:

26447-40-5

Molecular formula:

C15H10N2O2

IUPAC Name:

1,1'-methylenebis(2-isocyanatobenzene)

Constituent 2

Reference substance name:

Benzene, 1,1'-Methylenbis(isocyanato)-

IUPAC Name:

Benzene, 1,1'-Methylenbis(isocyanato)-

Details on test material:

- Name of test material (as cited in study report): Desmodur VP PU 1806
- Substance type: reddish liquid
- Physical state: 99.96%
- Expiration date of the lot/batch: 01 Nov 1990
- Storage condition of test material: 4 °C, under N2

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Borchen
- Age at study initiation: males 8 weeks and females 10 weeks
- Mean body weight at study initiation: males 184 g and females 167 g
- Fasting period before study: About 16 hours before and up to 4 hours after application
- Housing: groups of 5 per cage
- Diet (e.g. ad libitum): standard diet Altromin 1324 pellets
- Water (e.g. ad libitum): tap water
- Acclimation period: 5 days


ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22±2
- Humidity (%): 50±10
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

peanut oil

Details on oral exposure:

DOSAGE PREPARATION
The substance was dissolved in Peanut oil on a magnetic stirrer at room temperature. It was administered at a constant application volume of 10 ml/kg bw. The test substance was formulated in the application medium immediately before administration.

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: mortality and clinical signs were observed several times on the day of administration and twice daily thereafter. Animals were weighed before administration and weekly thereafter.
- Necropsy of survivors performed: yes

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

No mortalities were observed

Clinical signs:

other: All animals had increased salivation 30 min after application. One hr after application, all animals were free of clinical signs.

Gross pathology:

no abnormalities detected