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EC number: 239-349-0 | CAS number: 15307-93-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- February 07 - March 01, 1995
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Well documented and reported study, conducted equivalent to internationally accepted technical guideline in recognized research organization.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 995
- Report date:
- 1995
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- Version / remarks:
- ,1984
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,6-dichloro-N-phenylaniline
- EC Number:
- 239-349-0
- EC Name:
- 2,6-dichloro-N-phenylaniline
- Cas Number:
- 15307-93-4
- Molecular formula:
- C12H9Cl2N
- IUPAC Name:
- 2,6-dichloro-N-phenylaniline
- Details on test material:
- Identification Code: PBS 3247
Generic/Trade name: Dichlordiphenylamin dest.
BatchNo.: EN 322165.36
Appearance: beige solid
Purity: not available*
Solubility (in water): 0.0063 g/l
Received: 17/10/94
Storage: room temperature
Stability : 12/95
*: The test substance is identified by the code number. Identification and characterization is guaranteed by the sponsor. Data for purity were not available or not generated and documented according to the rules of Good Laboratory Practices.
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Composite samples (about 150 ml per concentration and control) of each test concentration were drawn by mixing identical volumes of the test solutions taken from the test vessels. SampIes of test solutions were taken immediately before exposure and after 72 hours exposure and stock solutions were taken 24 hours before exposure. All samples were kept at - 18°C to - 22°C until analysis.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- (Test) Water: composition according to the guideline
- Method: Stock solution: 100.4 mg test substance were mixed with 1.0 ml of a 0.2% TWEEN 80 solution in water (w/w) by a rubbowl and this suspension was then made up to 1000 ml with water. Calculated amounts of the stock solution to produce the desired test concentrations and vehicle to achieve identical vehicle concentrations were given into the water and were homogeneously distributed. The test solutions were equilibrated for 24 hours under test conditions.
- Controls: blank (test water) and vehicle control
- Chemical name of vehicle: polyoxy-ethylene-sorbitan-monooleate (TWEEN 80)
- Concentration of vehicle in test medium: stock solution: 2 mg/l, final test solutions including vehicle control: 0.116 mg/l
- Evidence of undissolved material: at nominal test concentrations 3.2 and 5.8 mg/l a deposit and some test substance on the surface of the test water were observed.
Test organisms
- Test organisms (species):
- Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)
- Details on test organisms:
- TEST ORGANISM
- Strain: 86.81 SAG
- Source (culture collection): Collection of algae cultures, Pflanzenphysiologisches Institut, University Nikolausberger Weg 18, 37073 Göttingen/Germany
- Age of inoculum (at test initiation): 3 days
- Method of cultivation: according to the guideline cited
ACCLIMATION
- Acclimation period: 3 days
- Culturing media and conditions: same as test
- Any deformed or abnormal cells observed: not reported
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
Test conditions
- Hardness:
- not indicated; the composition of the test water was according to the guideline
- Test temperature:
- 24 ± 1 °C
- pH:
- test start: 8.0
test end: 8.1 - 9.1 - Dissolved oxygen:
- not applicable
- Salinity:
- not applicable (freshwater)
- Nominal and measured concentrations:
- nominal concentration: 0.10, 0.18, 0.32, 0.58, 1.0, 1.8, 3.2, and 5.8 mg/l
measured at test start: 0.04, 0.09 ,0.19 ,0.28 ,0.41 ,0.99 ,0.98, and 2.97 mg/l
measured at test end: 0.04, 0.06, 0.12, 0.17, 0.31, 0.70, 1.28, and 2.02 mg/l
arithmetric mean: 0.04, 0.08, 0.16, 0.23, 0.36, 0.85, 1.13, and 2.50 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: 100 ml Erlenmeyer flasks, cotton stoppers, 50 ml test solution per flask
- Type (delete if not applicable): open
- Aeration: agitation on a lab shaker
- Initial cells density: 10700 cells/ml
- Control end cells density:
blank: 2'029'000 cells/ml
vehicle: 1'676'000 cells/ml
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 6
- No. of vessels per vehicle control (replicates): 6
GROWTH MEDIUM
- Standard medium used: yes
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: composition according to the guideline
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature was continuously measured; pH was measured at 0h and 72h exposure
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: Continuous illumination
- Light intensity and quality: cold white fluorescent light, 113 µE/m2sec (approx. 8000 lux)
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : cell density after 24, 48 and 72 hours exposure
- Determination of cell concentrations: cell counter
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: results not reported - Reference substance (positive control):
- not required
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.62 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Remarks on result:
- other: 95% CL: 0.58-0.66
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.23 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.28 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- (area under growth curves)
- Remarks on result:
- other: 95% CL: 0.27-0.30
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.08 mg/L
- Nominal / measured:
- meas. (arithm. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Remarks:
- (area under growth curves)
- Details on results:
- - Exponential growth in the control (for algal test): yes
- Any stimulation of growth found in any treatment: at nominal test concentrations of 0.04 and 0.09 mg/l, slight stimulation of growth was observed (5.6-7.5% stimulation for biomass and 1.4% for growth rate, respectively). - Reported statistics and error estimates:
- The EC-50 values were calculated according to the maximun likelihood method, logit model (Mc Cullagh, P., NeIder, J.A., 1983: Generalized linear models, Chapman & Hall, London).
slope: 2.4 for ErC50, 3.8 for EbC50. NOEC values were calculated on a 5% level.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- Criteria of OECD Guideline 201 (1984): Increase of the cell concentrations in the control cultures by a factor of at least 16 within three days).
- Conclusions:
- 72h-EC50: >0.1 ≤1 mg/l
- Executive summary:
In a 96-h toxicity study, green algae (Scenedesmus subspicatus) were exposed to PBS 3247 in accordance with OECD Guideline 201(Freshwater Alga and Cyanobacteria, Growth Inhibition Test) of 1984. The study was performed in compliance with GLP. Reliability grade 1 was assigned. Nominal concentrations of 0 (control and solvent control), 0.10, 0.18, 0.32, 0.58, 1.0, 1.8, 3.2, and 5.8 mg/l were tested. The toxicity endpoints were calculated based on arithmetic mean concentrations of 0.04, 0.08, 0.16, 0.23, 0.36, 0.85, 1.13 and 2.50 mg/l:
Inhibtion (growth rates):
ErC50 (0 - 72 h):
0.62 mg/l
95 % confidence limit:
0.58-0.66
slope:
2.4
NOErC ( 0-72 h):
(5 % level)
0.23 mg/I
Inhibition (areas under growth curves):
EbC50 (0 - 72 h):
0.28 mg/I
95 % confidence limit:
0.27-0.30
slope:
3.8
NOEbC (0-72 h):
(5 % level)
0.08 mg/l
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