Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 210-765-4 | CAS number: 623-03-0
Endpoint summary
Administrative data
Description of key information
For aquatic toxicity results on short-term toxicity to fish and invertebrates as well as results on toxicity to algae and microorganisms are available.
Fish:
In the 48-h acute toxicity study according to DIN 38 412, Leuciscus idus ('Goldorfe') the 48-h acute toxicity LC50 of 4-chlorobenzonitrile was determined to be 22 mg/L (Hooftmann et al., 1990). The 24-h LC50 and NOEC values, based on mortality, were 24 and 18 mg/L, respectively. Due to undissolved test material that could be found at the bottom of the test vessels at 18 mg/L and in addition at the water surface at 56 mg/L, mortality that occured at concentrations of 18 mg/L and higher may be attributed to the interaction of the undissolved test material with the test organisms.
The 48-h LC50 for fathead minnow, calculated in a QSAR approach (Zhao et al., 1993), was determined to be 14.4 mg/l. This value is lower than the calculation of the 96-h LC50 of 4-chlorobenzonitrile for fish of 61 mg/L using EPISUITE 3.12 (US-EPA 2004; reported in BUA Stoffbericht 265, 2006).
Invertebrates:
Due to the results of a test according to OECD 202, the EC50 (48 h) of 4-Chlorobenzonitrile for immobilisation was determined to be 16.1 mg/L (nominal) and 8.06 mg/L (actual) of test item. The corresponding NOEC (48 h) was 6.25 mg/L (nominal) and 2.02 mg/L (actual).
Algae:
Significant inhibitory effects for 4-Chlorobenzonitrile were observed at 50.0 and 100 mg/L (nominal) for growth rate and yield, respectively. Therefore the overall LOEC was determined to be 50.0 mg/L (nominal) and the overall NOEC was 25.0 mg/L (nominal) for growth rate and yield. The 72h-EC50 value for growth rate (ErC50) was 51.9 mg/L (nominal). The 72h-EC50 value for yield (EyC50) was 20.5 mg/L (nominal).
Microorganisms:
A toxicity threshold value of 4-chlorobenzonitrile of 50.6 mg/L for Pseudomonas putida was determined. The ‘Bewertungszahl' (Assessment figure or value for bacterio toxicity) is therefore 4.3 (Bewertung wassergefährdender Stoffe, 1979; Coenen, 1990).
The EC10 and EC50 of 4-chlorobenzonitrile as well as the NOEC and LOEC values were calculated by using probit analysis and the Williams-Test (BUA Stoffbericht 265, 2006), respectively, on the data presented by Coenen, 1990. The following results were presented:
- EC10 (18 h) = 52 mg/L
- EC50 (18 h) = 138 mg/L
- LOEC (18 h) = 52 mg/L
- NOEC (18 h) = 26 mg/L
In addition the EC50 of 4-chlorobenzonitrile was determined in a Microtox-test for the bioluminescent marine bacterium Vibrio fisheri.The EC50 was determined to be 8.7 mg/L.
The toxicity of 4-chlorobenzonitrile to the freshwater ciliate Tetrahymena pyriformis was determined in a 48-h TETRATOX-Test (results reported in (BUA Stoffbericht 265, 2006).
The 48-h EC50 was determined to be 137 mg/L (nominal concentration).
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
