Registration Dossier
Registration Dossier
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EC number: 205-736-8 | CAS number: 149-30-4
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP study; acceptable documented study report which meets basic scientific principles with limitations concerning the tester strains used, which are not in accordance with current guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 984
Materials and methods
- Principles of method if other than guideline:
- Method: other ames assay
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Benzothiazole-2-thiol
- EC Number:
- 205-736-8
- EC Name:
- Benzothiazole-2-thiol
- Cas Number:
- 149-30-4
- Molecular formula:
- C7H5NS2
- IUPAC Name:
- 1,3-benzothiazole-2-thiol
- Details on test material:
- mercaptobenzothiazole, lot #39-14B
Constituent 1
Method
- Target gene:
- ames assay
Species / strain
- Species / strain / cell type:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9-mix
- Test concentrations with justification for top dose:
- pre-test: 0, 100, 333, 1000, 3333, 10000 µg/plate; main experiment: 0, 3, 10, 30, 100, 300 µg/plate
up to 300 ug/plate; second experiment: +S9 TA 1537, TA98: 0, 100, 250, 300, 450, 600 µg/plate
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: -S9: sodium azide, 9-aminoacridine, 2-nitrofluorene, +S9: 2-aminoanthracene
- Details on test system and experimental conditions:
- IUCLID4 Type: Ames test
Results and discussion
Test results
- Species / strain:
- S. typhimurium, other: TA98, TA100, TA1535, TA1537, TA1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'. Remarks: TA98, TA100, TA1535, TA1537, TA1538
Any other information on results incl. tables
Toxicity determined in a pre-test: toxicity indicated at 333 µg/plate and higher
The test substance was negative in the ames assay with and without metabolic activation.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
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