.beta.-Alanine, N-[5-(acetylamino)-4-[2-(2-chloro-4,6-dinitrophenyl)diazenyl]-2-methoxyphenyl]-, 2-oxo-2-(phenylmethoxy)ethyl ester

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From August 14th to September 5th, 2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

1992

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Blue REg 6080

IUPAC Name:

Blue REg 6080

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

Due to the very low water solubility of the test item, a supersaturated stock dispersion of the test item with a loading rate of nominal 100 mg/I was continuously stirred at room temperature in the dark over 96 hours. Then the dispersion was filtered. The undiluted filtrate with the maximum concentration of dissolved test item was used as the test medium.
Additionally, a control was tested in parallel.

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

7 fish in control
7 fish in test item concentration

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

250 mg CaCO3/l

Test temperature:

23 °C

pH:

7.8-8

Dissolved oxygen:

>= 8 mg/l

Nominal and measured concentrations:

Nominal 100 mg/l as loading rate

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 2

Duration:

96 h

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

The 96-hour NOEC (highest concentration tested without toxic effects after the exposure period of 96 hours), and the 96-hour LC0 of test item to zebra fish were determined to be at least at a loading rate of 100 mg/I. The 96-hour NOEC and the 96-hour LC0 might even be higher than this loading rate, but loading rates above 100 mg/I were not tested, according to the guidelines.
The 96-hour LOEC (lowest concentration with toxic effects) and the 96-hour LC50 were clearly higher than the loading rate of 100 mg/I. These values could not be quantified, since the test item had no toxic effect up to the highest concentration which could be dissolved in the test water.

The biological results are related to the loading rates of 100 mg/l. No effects were observed at the solubility limit of < 0.8 µg/l (detection limit). In conclusion, the LC50 is > 0.8 µg/l, the NOEC 0.8 µg/l.

Applicant's summary and conclusion

Conclusions:

Test item has no toxic effect on the fish up to its solubility limit in test water.