Zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate)

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

13 May - 27 May 1992

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP-Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Report date:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

Ministerium für Umwelt Baden-Württemberg, Stuttgart, Germany

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate)

EC Number:

230-257-6

EC Name:

Zinc O,O,O',O'-tetrabutyl bis(phosphorodithioate)

Cas Number:

6990-43-8

Molecular formula:

C16H36O4P2S4Zn (neutral monomer)

IUPAC Name:

zinc bis(O,O-dibutyl dithiophosphate)

Details on test material:

- Name of test material (as cited in study report): Zn-di-n-butyl dithiophosphate
- Physical state: light yellow liquid
- Analytical purity: ca. 92%
- Impurities (identity and concentrations): paraffinic mineral oil, ca. 8%
- Lot/batch No.: 106 92 78
- Expiration date of the lot/batch: June 1992
- Stability under test conditions: stable
- Storage condition of test material: no special storage conditions

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Interfauna, Süddeutsche Versuchstierfarm, Tuttlingen, Germany
- Weight at study initiation: 182-198 g (males), 168-186 g (females)
- Housing: individually in Macrolon cages (area 800 cm², height 17 cm)
- Diet: Haltungsdiät "ALMA 0801 H 1003", twice 8 g daily
- Water: ad libitum
- Acclimation period: 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-22
- Humidity (%): 30-70
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

5

Control animals:

other: not required

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: animals were observed daily for mortality and clinical signs. Body weights were determined prior to study initiation, on the day of oral administration (Day 0), and daily thereafter, except on Days 4, 11 and 12 post-administration.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross necropsy

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

No mortality occurred during the study period.

Clinical signs:

other: Two rats (one male and one female) were slightly apathetic on the day of application (Day 0) and on Day 1 post-application. The anal region of two other male rats was slightly covered with light brown and pasty excrements on Day 1 post-application. On Day

Gross pathology:

No macroscopical organ changes were observed in any animal.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

CLP: not classified
DSD: not classified