1H-Imidazolium, 3-ethyl-1-methyl-, methanesulfonate (1:1)

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: OECD guideline study conducted according to GLP.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD 431 plus 439

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

1-ethyl-3-methyl-1H-imidazol-3-ium methanesulfonate

EC Number:

604-453-0

Cas Number:

145022-45-3

Molecular formula:

C6 H11 N2. C H3 O3 S

IUPAC Name:

1-ethyl-3-methyl-1H-imidazol-3-ium methanesulfonate

Test animals

Species:

other: EpiDerm™ tissue samples

Test system

Type of coverage:

open

Vehicle:

unchanged (no vehicle)

Duration of treatment / exposure:

irritation test: 1 h plus 42 hrs post treatment incubation
corrosion test: 3 min and 1 h, respectively

Results and discussion

Any other information on results incl. tables

irritation test

	exposure 1 h plus 42 hrs post-treatment incubation
viability (% negative control)	95
corrosion test
	exposure: 3 min	exposure: 1 h
viability (% negative control)	88	82

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information

Conclusions:

EMIM Methansulfonat does not show a skin irritation potential in the EpiDerm™ skin corrosion/irritation test.

Executive summary:

The potential of EMIM Methansulfonat to cause dermal corrosion/irritation was assessed by a single topical application of 50 μL (corrosion test) or 30 μL (irritation test) of the test substance to a reconstructed three dimensional human epidermis model (EpiDerm™). For the corrosion test two EpiDerm™ tissue samples were incubated with the test substance for 3 minutes and 1 hour, respectively. The irritation test was performed with three EpiDerm™ tissue samples, which were incubated with the test substance for 1 hour followed by a 42-hours post-incubation period. Tissue destruction was determined by measuring the metabolic activity of the tissue after exposure/post-incubation using a colorimetric test. The reduction of mitochondrial dehydrogenase activity, measured by reduced formazan production after incubation with a tetrazolium salt (MTT) was chosen as endpoint. The formazan production of the testsubstance treated epidermal tissues is compared to that of negative control tissues. The quotient of the values indicates the relative tissue viability. The EpiDerm™ skin corrosivity/irritation test showed the following results: The test substance is not able to reduce MTT directly. Based on the observed results and applying the evaluation criteria it was concluded, that EMIM Methansulfonat does not show a skin irritation potential in the EpiDerm™ skin corrosion/irritation test under the test conditions chosen.