Alcohols, C18-22, reaction products with maleic anhydride and sodium bisulfite

Short-term toxicity to aquatic invertebrates

Description of key information

 IC50  > 100 mg/L of active substance 
 NOEC = 100 mg/L of active substance 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

100 mg/L

Additional information

In the acute immobilisation test withDaphnia magna(STRAUS), conducted as a non GLP screening test, the effects of the test item Sulfosuccinate of C18-22 Alkyl-Alcohol were determined according to OECD 202 (2004).

The test item is a pasty liquid and emulsifiable in water. The study was conducted with three concentration levels of the test item around the critical micelle concentration (CMC) of 375 ± 25 µg/L. The selected test item concentrations were 0.100 - 1.00 - 10.0 mg/L. The test concentrations were prepared from a stock solution of 10.0 mg/L by serial dilution with dilution water. The stock solution was homogenised by stirring with approximately 1100 rpm for 1 hour at room temperature. The concentration levels of 0.100 and 1.00 mg/L were visually clear throughout the exposure. The concentration level of 10.0 mg/L contained fine, homogeneously dispersed particles of the test item, which precipitated at the end of the exposure (after 24 hours). Twenty daphnids were exposed to each concentration level and the control.

No analytical monitoring of the tested solutions was requested by the sponsor, but samples of all tested concentration levels and the control were taken and stored at -20±2 °C for possible analyses.

No effects were observed on Daphnia magna in any of the tested concentration levels in the range of 0.100 to 10.0 mg/L throughout the testing period

The study was performed at a concentration much higher than the solubility level of the substance and the Critical Micell Concentration, therefore it can be assumed that the representative value of 100 mg/L estimated and confirmed by the similar substance Lanoline sulphosuccinate is reliable

The acute toxicity of the test item Sulfosuccinate of lanolin Alcohol to Daphnia magna was determined in a 48‑hour full static test according to the OECD Guideline for Testing of Chemicals, No. 202 (2004)

For this purpose, juvenile daphnids (< 24 hours old at initiation of the test) were exposed to an aqueous test medium containing the active ingredient at five different concentrations, namely 4.3, 9.4, 20.7, 45.5 and 100.0 mg/L, corresponding to 14.2, 31.3, 68.9, 151.5 and 333.3 mg/L as test item.

The exposed organisms were checked for immobilization 24 and 48 hours after test initiation.

The pH of the test media after preparation was in the range 7.27 – 7.51 for test item solutions and 7.29 for negative control.

The incubation temperature was in the range 18.7 – 21.2 °C.

Light intensity during the 48 hours of test period was in the range 780 - 797 Lux.

At the end of test period, in the negative control 0 % of immobilization was observed and no daphnid showed signs of disease or stress (e.g. to be trapped on the test water surface). These values comply with the validity criteria of the test, that provide a maximum immobilization or a maximum number of daphnids showing disease or stress signs of 10 % in the negative control medium at the end of the test.

The obtained experimental results allowed to calculate the IC₀, IC₁₀₀ the IC₅₀values and the NOEC at 24 and 48 hours.

The IC50 was defined > 100 mg/L of active substance (> 333.3 mg/L of test item), the NOEC 100 mg/L of active substance ( 333.3 mg/L nominal concentration of the test item).