3,4-dichloroaniline

Toxicity to terrestrial plants

Administrative data

Endpoint:

toxicity to terrestrial plants: short-term

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study peer reviewed in EU-Risk assessment

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Phytotoxicity studies with Lactuca Sativa in soil and nutrient solution

Author:

Hulzebos et al.

Year:

1993

Bibliographic source:

Environ. Toxicol. Chem. 12(6), 1079-1094

Reference 2

Reference Type:

secondary source

Title:

3,4-dichloroaniline (3,4-DCA)

Author:

EU-Risk Assessment Report

Year:

2006

Bibliographic source:

EUR 22235 EN; ISSN 1018-5593

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

3,4-dichloroaniline

EC Number:

202-448-4

EC Name:

3,4-dichloroaniline

Cas Number:

95-76-1

Molecular formula:

C6H5Cl2N

IUPAC Name:

3,4-dichloroaniline

Details on test material:

- Name of test material (as cited in study report): 3,4-Dichloranilin,
- Purity: >= 95 %

Sampling and analysis

Analytical monitoring:

yes

Test substrate

Vehicle:

no

Test organisms

Species:

Lactuca sativa

Plant group:

Dicotyledonae (dicots)

Study design

Test type:

early seedling growth toxicity test

Study type:

laboratory study

Substrate type:

natural soil

Limit test:

no

Total exposure duration:

14 d

Test conditions

Test temperature:

21 +/- 4 °C

pH:

7.5

Nominal and measured concentrations:

Nominal concentrations from 0.32 to 100 mg/kg dw soil were spaced by a factor of 3.2.

Results and discussion

Effect concentrations

Effect concentrations 1

Species:

Lactuca sativa

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

> 10 - < 32 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 2

Species:

Lactuca sativa

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

> 10 - < 32 mg/kg soil dw

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Effect concentrations 3

Species:

Lactuca sativa

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

1.7 other: mg/L nutrient solution

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth

Remarks on result:

other: Day 16 and 21

Any other information on results incl. tables

The test was performed according OECD Guideline 208. Nominal concentrations from 0.32 to

100 mg/kg dw soil were spaced by a factor of 3.2. Duplicate trays were used for the control and

at least three test concentrations. Immediately after DCA treatment seeds were put into the soil.

After four days, only the first five germinated seedlings were required, and any additional

seedlings were discarded in order to exclude the effect of the binding of 3,4-DCA to soil

organics. Seeds could possibly have germinated after four days as 3,4-DCA was less available

and this effect was outside of the scope of this test. After 7 and 14 days, shoots were harvested

by cutting them off at soil level. The fresh weight of each plant was determined immediately

after harvesting. Water content and pH of the soil were checked at the start and at the end of the

experiment. The concentration of 3,4-DCA was analysed by extraction with toluene, followed by

analysis of the extract. The concentration of 3,4-DCA has dropped to a low value (< 30%) of the

initial concentration. The EC50 obtained for 3,4-DCA is slightly above 10 mg/kg soil dw. One

concentration higher (32 mg/kg dry soil), seeds did not germinate, and therefore it is set equal to

10.

Additionally the result in nutrient solution test:

16-21-day EC50 = 1.7 mg/l nutrient solution

Effect: growth (fresh weight), organic matter 1.4%, pH 7.5, nominal concentration.