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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to EU / OECD guideline.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
methyl 4-cyano-2-nitrobenzoate
EC Number:
610-840-5
Cas Number:
52449-76-0
Molecular formula:
C9H6N2O4
IUPAC Name:
methyl 4-cyano-2-nitrobenzoate

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
yes
Details on test solutions:
vehicle = dilution water

Test organisms

Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Hardness:
Total hardness: 40 - 180 mg CaC03/L
Test temperature:
23°C +/- 2 °C
pH:
6.0 - 8.0
Dissolved oxygen:
The dissolved oxygen concentration was more than 80 % of the air saturation value.
Nominal and measured concentrations:
Test concentrations. 6.25- 12.5- 25- 50- 100 mg/L (nominal concentrations)
Reference substance (positive control):
no
Remarks:
No reference item is recommended for this lest according to the guideline.

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
ln this study the test substance was found to be toxic to Zebra fish after 96 h at nominal concentrations of 12.5 mg/L and greater.
The LC50 (96 h) was 14 (12- 15) mg/L, the NOEC after 96 h was 6.25 mg/L.