2,3-epoxypropyl phenyl ether

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: genome mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with restrictions: E. coli was not included to detect cross-linking, no data on cytotoxicity

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 471 (Bacterial Reverse Mutation Assay)

Deviations:

yes

Remarks:

E. coli was not included to detect cross-linking, no data on cytotoxicity

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

phenyl glycidyl ether

IUPAC Name:

phenyl glycidyl ether

Details on test material:

- Name of test material (as cited in study report): phenyl glycidyl ether
No further information.

Method

Species / strain

Species / strain / cell type:

other: S. typhimurium TA 1535, TA 97, TA 98 and TA 100

Metabolic activation:

with and without

Metabolic activation system:

S9 fraction from Aroclor 1254-induced male Sprague-Dawley rat or Syrian hamster liver

Test concentrations with justification for top dose:

0, 3, 10, 33, 100, 166, 333, 666, 1000 µg/plate (TA1535, TA97 and TA100)
0, 10, 33, 100, 333, 1000 µg/plate (TA98)

Controls

Untreated negative controls:

not specified

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: with S9: 2-aminoanthracene (all strains); without S9: 2-nitrofluorene (TA98), sodium azide (TA100, TA1535), 9-aminoacridine (TA97),

Details on test system and experimental conditions:

METHOD OF APPLICATION: preincubation

DURATION
- Preincubation period: 20 Min.

Evaluation criteria:

No data.

Results and discussion

Test results

Test results 1

Species / strain:

other: S. typhimurium TA 1535, TA 97 and TA 100

Metabolic activation:

with and without

Genotoxicity:

positive

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Test results 2

Species / strain:

S. typhimurium TA 98

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

valid

Untreated negative controls validity:

not specified

Positive controls validity:

valid

Remarks on result:

other: all strains/cell types tested

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Table 1:

Strain: TA100
Dose	No Activation		No Activation		10% HLI		10% HLI		10% RLI		10% RLI
	(Positive)		(Positive)		(Positive)		(Positive)		(Positive)		(Positive)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	143	3.2	119	14.2	125	8.6	123	7.2	123	3.7	112	7.1
3			138	1.2
10	193	10.5	171	7.5	140	14.5			124	7.5
33	342	16.5	278	22.8	131	10.6			122	17.3
100	672	23.9	519	13.7	153	11.8	137	12	142	20.4	145	11.3
166							158	7.8			153	8.6
333	1427	52.5	882	24.9	139x	72.9	232	18.5	297	18.2	259	15.1
666							439	25.2			494	19.1
1000	1984	261.9			701	94.5	619	35.6	1087	53.4	657	16.6
Positive Control	694	18.1	368	15.8	1329	24.8	501	75.1	1007	35.3	689	15.1
Strain: TA1535
Dose	No Activation		No Activation		10% HLI		10% HLI		10% RLI		10% RLI
	(Positive)		(Positive)		(Positive)		(Positive)		(Positive)		(Positive)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	41	1.3	18	2	14	2.3	10	2	11	0.6	9	0.3
3			22	2.8
10	105	8.2	31	2.8	11	2.6			13	2.5
33	225	3.8	89	5.5	10	1.5			13	2.8
100	463	20.2	193	7.1	14	4.2	15	1.3	21	3.3	13	0.9
166							12	0.9			18	2.8
333	826	69.2	473	57.3	26	3.2	30	4.3	63	9.2	50	3
666							157	4.6			187	11.8
1000	738	122.2			280	15.1	290	20.8	424	44.6	329	15.6
Positive Control	795	12.9	267	12.5	431	5.5	259	7.8	291	3.4	126	6.9
Strain: TA97
Dose	No Activation		No Activation		10% HLI		10% HLI		10% RLI		10% RLI
	(Positive)		(Positive)		(Positive)		(Positive)		(Positive)		(Positive)
Protocol	Preincubation		Preincubation		Preincubation		Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	120	19.9	132	2.6	177	1.2	156	3.5	181	4.2	155	8.4
3			168	5.4
10	185	16.3	179	8.4	216	17.4			196	9.9
33	230	3.2	225	13.9	196	14.6			199	18.4
100	338	4.9	343	16.4	212	6.4	171	12.9	215	14.3	209	4.2
166							199	14.8			212	17.9
333	658	76.2	614	44.4	246	10.7	233	12.8	291	3.7	277	17.9
666							403	18.6			494	14.8
1000	21	8.8			746	15.6	605	24.4	872	59.1	697	37.2
Positive Control	1514	22.1	364	23	910	42.6	546	10	666	36.9	436	6.4
Strain: TA98
Dose	No Activation		10% HLI		10% RLI
	(Negative)		(Negative)		(Negative)
Protocol	Preincubation		Preincubation		Preincubation
ug/Plate	Mean	± SEM	Mean	± SEM	Mean	± SEM
0	26	4.6	43	6.8	32	4.4
10	29	4	53	2	29	4.9
33	29	6.2	46	1.5	35	5.5
100	30	4.9	45	5	36	3.3
333	33	2.7	46	1	34	1.2
1000	31	3.3	52	5	40	3.5
Positive Control	973	20.8	1061	80.4	643	2.3
Abbreviations:
RLI = induced male Sprague Dawley rat liver S9
HLI = induced male Syrian hamster liver S9
s = Slight Toxicity; p = Precipitate; x = Slight Toxicity and Precipitate; T = Toxic; c = Contamination