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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study conducted in accordance with GLP.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2005
Report date:
2005

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Retinyl propionate
EC Number:
230-363-2
EC Name:
Retinyl propionate
Cas Number:
7069-42-3
Molecular formula:
C23H34O2
IUPAC Name:
retinyl propionate
Details on test material:
- Vitamin A-propionat, (retinyl-propionate)
- Purity: 95.9 w -%
- Batch No.: 00000777LO PBG-Nr.10540158
- Water solubility: < 10 ug/L at 20.0 °C ± 0.1 °C

Sampling and analysis

Analytical monitoring:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
- Laboratory culture: activated sludge obtained from a laboratory wastewater plant treating municipal sewage
- Initial biomass concentration: 1 g/L dry matter

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
180 min

Test conditions

Test temperature:
20 ± 2 °C
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, total volume, test volume: Erlenmeyer-vessel, 250 ml, 250 ml
- Synthetic medium: 8 ml/vessel 100-fold concentrated OECD medium
- Oxygen concentration during aeration: > 2.5 mg/l
- Oxygen concentration immediately before measurement: > 6.5 mg/l
- Duration of the measurement of oxygen consumption: 8 - 10 min


Reference substance (positive control):
yes
Remarks:
3,5-Dichlorophenol (500 mg/L)

Results and discussion

Effect concentrationsopen allclose all
Duration:
180 min
Dose descriptor:
other: EC20
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
180 min
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Results with reference substance (positive control):
Effect concentrations after 180 min (nominal):
EC20 = ca. 1.2 mg/L
EC50 = ca. 18 mg/L
EC80 = ca. 50 mg/L

Applicant's summary and conclusion

Validity criteria fulfilled:
yes