1-amino-4-(3-amino-2,4,6-trimethyl-5-sulphoanilino)-9,10-dihydro-9,10-dioxoanthracene-2-sulphonic acid

Endpoint summary

Description of key information

The acute oral LD 50 in the rat was determind to be >2000 mg/kg bw (males and females). For acute dermal and acute inhalation toxicity no data are currently available.

Key value for chemical safety assessment

Acute toxicity: via oral route

Link to relevant study records

Reference

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

not applicable

GLP compliance:

no

Test type:

fixed dose procedure

Limit test:

yes

Species:

rat

Strain:

Wistar

Remarks:

Albino rat

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Females (if applicable) nulliparous and non-pregnant: [yes]
- Age at study initiation: no data (adult)
- Weight at study initiation: no data
- Fasting period before study: yes
- Housing: groups of 3
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: no data

ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: To:

Route of administration:

oral: gavage

Vehicle:

not specified

Details on oral exposure:

single exposure

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

3 males and 3 females

Control animals:

no

Details on study design:

no data

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Mortality:

no deaths occurred

Clinical signs:

other: Piloerection, hunched posture, dyspnoea, diarrhea observed on the day of TS application

Gross pathology:

No clinical findings noted.

Other findings:

Extremities showed blue discolouration. Animals recovered fully within 6 days.

Interpretation of results:

GHS criteria not met

Conclusions:

LD 50 > 2000 mg/kg bw.

Executive summary:

The test substance is judged to harvest no acute orla toxicity to rats.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed

Dose descriptor:

LD50

Value:

2 000 mg/kg bw

Additional information

LD50 to both sexes: >2000 mg/kg bw.

LD50 to male rats: >2000 mg/kg bw.

LD50 to female rats: >2000 mg/kg bw.