1,8-naphthylenediamine

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

Version / remarks:

Enrichment test of a chemical substance in the body of the fish and shellfish

Deviations:

not specified

Principles of method if other than guideline:

Method: semi-static

GLP compliance:

yes

Remarks:

audits and inspections carried out by department of the Foundation Chemicals Inspection Association chemical safety center Kyushu laboratory

Specific details on test material used for the study:

Test substance number K-602B
Purity: 100%
Identification via infrared spectroscopy, mass spectrometry and nuclear magnetic resonance spectroscopy
The test substance was confirmed to be stable under storage conditions and test conditions.

Radiolabelling:

not specified

Vehicle:

yes

Remarks:

dimethyl sulfoxide

Details on preparation of test solutions, spiked fish food or sediment:

Preparative method:
- First concentration group: 0.8 g test substance dissolved in dimethyl sulfoxide was added dropwise to 8 L deionized water to prepare a stock solution of 100 mg/L.
- Second concentration group: 0.08 g test substance dissolved in dimethyl sulfoxide was added dropwise to 0.8 L deionized water to prepare a stock solution of 100 mg/L.
- Control: 80 mg Methyl sulfoxide was dissolved in deionized water.

Test organisms (species):

Cyprinus carpio

Details on test organisms:

- Test fish: carp Cyprinus carpio
- Entry date: 1988-11-15
- Supplier: Sugishima fish farm (Yubinbangoa 66 Kumamoto Prefecture Yatsushiro Gunchikuichiban cho 123-2)
- Conditions for fish keeping: At reception, fish showing visible abnormal signs were removed and remaining ones kept for 32 days in running water at 25 +/- 2 °C..
- External disinfection: External disinfection was performed according to static condition for about 24 hours in a aqueous solution containing 50 mg/L Terramycin (from Pfizer) and 7 g/L sodium chloride.
- Weight: 22.1 g (average)
- Lenght: about 9.6 cm
- Lipid content: 3.8 %
- Feed: Pelleted feed for carp (Manufacturer: Nippon Formula Feed Mfg Co., Ltd.)
- Feeding amount and interval: Amount corresponding to about 2 % of the total body weight of test fish was fed twince a day by halves. On the day of fish sampling feed was not supplied.

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Remarks:

Ground water pumped at Kyushu laboratory site

Total exposure / uptake duration:

6 wk

Test temperature:

25 °C +/- 2 °C

Dissolved oxygen:

first concentration group: 6.2-7.6 mg/L;
second concentration group: 6.2-7.6 mg/L
control: 7.1-7.9 mg/L

Details on test conditions:

- Test start: 1989-01-20
- Exposure start date: 1989-01-20
- Exposure end date: 1989-03-3
- Test end: 1989-03-9
- Test tank: Glass tank of 100 litre in volume
- Test water: 2 ml stock solution and 800 ml test water
- Number of fisch (at initiation of exposure): 15 fishes
- Number of fish in control group (at initiation of exposure): 7 fishes
- Duration of exposure: 6 weeks

Nominal and measured concentrations:

first concentration: 0.1 mg/L
second concentration: 0.01 mg/L

Reference substance (positive control):

not specified

Lipid content:

3.8 %

Time point:

other: Λ v.

Type:

BCF

Value:

3.6 dimensionless

Basis:

not specified

Remarks on result:

other: 3.6 ~ 6.1

Remarks:

Conc.in environment / dose:0.1 mg/L

Type:

BCF

Value:

< 3.7 dimensionless

Basis:

not specified

Remarks on result:

other: <3.7 ~ 6.5

Remarks:

Conc.in environment / dose:0.01 mg/L

Reported statistics:

Recovery rate
- Test water analysis: 89.6%, 87.7% average 88.6%
- Test fish analysis: 72.1% 75.2% average 73,6%

Validity criteria fulfilled:

not specified

Conclusions:

The BCF to 1,8-naphthylenediamine was calculated for a concentration of 0.1 mg/L at 3.6 ~ 6.1 and for a concentration of 0.01 mg/L at <3.7 ~ 6.5.

Executive summary:

The BCF to 1,8-naphthylenediamine was calculated for a concentration of 0.1 mg/L at 3.6 ~ 6.1 and for a concentration of 0.01 mg/L at <3.7 ~ 6.5.

Description of key information

The BCF to 1,8-naphthylenediamine was calculated to be 3.6 ~ 6.1 for a concentration of 0.1 mg/L and <3.7 ~ 6.5 for a concentration of 0.01 mg/L.

Key value for chemical safety assessment

BCF (aquatic species):

6.5 dimensionless