1-chloromethyl-4-fluoro-1,4-diazoniabicyclo[2.2.2]octane bis(tetrafluoroborate)

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1992

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: In alternative Methods in Toxicology, Vol. 6, progress in-Vitro Toxicology, pp.131-138

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

414-380-4

EC Name:

-

Cas Number:

140681-55-6

Molecular formula:

C7H14B2ClF9N2

IUPAC Name:

1-(chloromethyl)-4-fluoro-1,4-diazabicyclo[2.2.2]octane-1,4-diium; bis(tetrafluoroboranuide)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

source: air products and chemicals, INC
date received: 01/08/92
test article label: selectflour(TM) Flourination Reagent, RD-591-11874-88
Storage: The test article was stored at ambient room temperature & humidity.
Test Article description: White Powder
sample preparation: the test article was diluted with distilled water to appropriate concentrations.

Test animals / tissue source

Species:

other: fertile DeKalb XL strain eggs

Details on test animals or tissues and environmental conditions:

Forty fertile DeKalb XL strain eggs was selected for use from a larger group received on 01/03/92 from Moyer's Chicks, quakertown, pennsylvania. the eggs were kept in incubators at 99±2℉ and 50-60% relative humidity and their position within the incubator changed daily.

Test system

Amount / concentration applied:

There're four groups.
Group No. 1: 0.05%
Group No. 2: 0.10%
Group No. 3: 0.50%.
Group No. 4: 1.00%.

Number of animals or in vitro replicates:

every group has 10 eggs.

Details on study design:

Upon received the eggs were placed in a commercial incubator for 14 days. During this 14 day period, the positioning of the eggs trays within the incubator was rotated daily, Monday through Friday, to ensure even atmospheric exposure.

On day 4 of the incubation period, the eggs were removed from the incubator and candled to determine the location of the embryo. After determining the presence and location of the embryo, a small hole was drilled into the narrow end of each egg and approximately 2.5ml of albumen was removed using a needle and syringe. The hole was then sealed with collodion adhesive. A rectangular window was then cut in through the shell directly over the developing embryo. All cutting was performed using a dentist’s drill with a diamond wheel bit. The rectangular window was removed with forceps and the opening covered with transparent tape and the egg returned to the incubator.

On day 14 the eggs were removed from the incubator, the tape peeled back and the chorioallantoic membrane(CAM) examined for any abnormalities. Any egg with improperly developed membranes, undeveloped membranes or any abnormality was discarded.

Following the pre-dose examination, a Teflon ring was gently placed on the CAM and 40 ul of the test article dilution was pipette into the ring. The window was then re-sealed, the egg numbered and then returned to incubator.

Results and discussion

Any other information on results incl. tables

Group No.1

Concentrations: 0.05%	Egg number
Observations	91	92	93	94	95	96	97	98	99	100
Normal	x	x		x	x	x	x	x	x
Ghost vessels			x							x

 

 

Group No.2

Concentrations: 0.10%	Egg number
Observations	31	32	33	34	35	36	37	38	39	40
Normal	x	x				x	x	x		x
Ghost vessels			x	x	x				x

 

 

Group No.3

Concentrations: 0.50%	Egg number
Observations	11	12	13	14	15	16	17	18	19	20
Normal						x	x			x
Ghost vessels	x	x	x	x	x			x	x

 

 

 

Group No.4

Concentrations: 1.00%	Egg number
Observations	81	82	83	84	85	86	87	88	89	90
Normal							x
Capillary injection						x
Ghost vessels	x	x	x	x	x	x		x	x	x
Minimal hemorrhage		x

Applicant's summary and conclusion

Interpretation of results:

other: Causes serious eye damage.

Executive summary:

The RC₅₀and 95% confidence Limited are: 0.18( 0.08-0.40)%. Based on the results of this study, the test article is an eye irritant.