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EC number: 202-928-3 | CAS number: 101-25-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09.07.2019 - 19.09.2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- EC Number:
- 202-928-3
- EC Name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- Cas Number:
- 101-25-7
- Molecular formula:
- C5H10N6O2
- IUPAC Name:
- 3,7-dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At the start of the exposure and at the renewal (0 and 24 hours), monitoring samples of the fresh media were taken after preparation of the limit concentration and analyzed.
At the renewal and at the end of the exposure (24 and 48 hours), samples of the 24-hours old media were taken directly from the test vessels and analyzed.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- The study was conducted under semi-static conditions over a period of 48 hours with the limit concentration of 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane. The limit concentration was treated with ultrasound for 5 minutes at room temperature. The limit concentration was visually clear throughout the exposure.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Daphnia magna STRAUS, obtained from continuous laboratory cultures.
Origin Institut für Wasser-, Boden- und Lufthygiene (WaBoLu), 14195 Berlin, Germany
Breeder Noack Laboratorien GmbH, Käthe-Paulus-Str. 1, 31157 Sarstedt, Germany
Culture In glass vessels (2 - 3 L capacity) with approximately 1.8 L culture medium, at 20 2 °C, in an incubator, 16 hours illumination; light
intensity of max. 1500 lx
Culture medium Elendt M4, according to OECD 202, Annex 3 (2004) is used.
Feeding of the culture stocks The daphnids were fed at least 5 times per week ad libitum with a mix of unicellular green algae, e.g. Pseudokirchneriella
subcapitata and Desmodesmus subspicatus. The algae were cultured at the test facility.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 224 - 267 mg CaCO3 /L
- Test temperature:
- 18 - 22 °C, constant within ± 1 °C
- pH:
- 7,5 - 8,86
- Dissolved oxygen:
- 8.41 mg/L - 8,98 mg/L
- Conductivity:
- 564 - 600 µS/cm
- Nominal and measured concentrations:
- Sampling date 0 hours 24 hours 24 hour 48 hours
Fresh medium old medium fresh medium old medium
Nominal
test item
concentration Meas. conc. Meas. conc. Meas. conc. Meas. conc.
[mg/L] [mg/L] % [mg/L] % [mg/L] % [mg/L] %
110 110 100 103 94 109 99 105 95
Control < LOQ < LOQ < LOQ < LOQ
Meas. conc. measured concentration of the test item, dilution and weighing factor of analytical stock solution taken into
account
% percent of the nominal concentration of the test item
LOQ limit of quantification (0.100 mg test item /L) - Reference substance (positive control):
- yes
Results and discussion
Effect concentrations
- Key result
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 110 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- Immobilization Rates after 24 and 48 hours of Exposure in the Definitive Test
(n = 20, divided into 4 replicates with 5 daphnids each)
Test substance (110 mg/L) 0 of 20
Control 0 of 20 - Results with reference substance (positive control):
- The percentage of immobility for the reference item potassium dichromate (SIGMA-ALDRICH, batch number MKBV0900V, purity 99.0%, CAS RN 7778-50-9) was determined after 24 hours from 2019-07-17 to 2019-07-18.
EC50 1,95 mg/L
95% confidence limits 1,00 - 4,00 mg/L
Valid range 0.6 - 2.4 mg/L, acc. to AQS P 9/2 (02/2000)
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the limit concentration 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane, no effects on Daphnia magna were observed.
- Executive summary:
In the limit concentration 110 mg/L of the test item 3,7-Dinitroso-1,3,5,7-tetraazabicyclo[3.3.1]nonane, no effects on Daphnia magna were observed.
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