Nickel, 5-[2-(2-hydroxy-3,7-disulfo-1-naphthalenyl)diazenyl]-1H-1,2,4-triazole-3-carboxylate sodium complexes

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

-

EC Number:

443-510-2

EC Name:

-

Cas Number:

738587-10-5

Molecular formula:

C26H14N10Ni2O18S4 as free acid

IUPAC Name:

dinickel(2+) sodium 3-carboxy-5-[(1E)-2-(7-oxido-2,6-disulfonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide 3-carboxy-5-[(1E)-2-(7-oxido-2-sulfo-6-sulfonatonaphthalen-1-yl)diazen-1-yl]-1H-1,2,4-triazol-1-ide

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

The concentrations of the test substance were measured at 0 and 48 hours using a spectrophotometric method. At the start of the test the excess test solutions were sampled and at the end of the test one replicate of the dilution water control and test concentration was sampled.

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Source: Obtained from continuous laboratory cultures at Brixham Environmental Laboratory
- Age at study initiation: <24h
- Method of breeding: Diploid parthenogenesis
- Feeding during test: none
- Food type: Daphnia stock cultures were fed Chlorella vulgaris & 'Microfeast'

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

240 mg CaCO3/L

Test temperature:

The thermometer readings at 0, 24 and 48h were 20.2-20.3 degree C. A continuous temperature recorded automatically over the 48h remained within 20 +/-1 degree C

pH:

7.96-8.03

Dissolved oxygen:

8.6-9.0 mg/L

Salinity:

Conductivity 669 uS/cm

Nominal and measured concentrations:

The limit of detection of the test substance was 0.18 mg/L. The overall mean measured concentration was 100% of the nominal values. The percentage loss in the mean measured concentrations over the test period was 8%.

The nominal concentrations were used for the calculation and reporting of results.

Details on test conditions:

TEST SYSTEM
- Test vessel: Borosilicate glass beakers covered with loose fitting covers
- Size, headspace, fill volume: 250ml nominal capacity containing 200 ml of test solution providing a depth of 60mm.
- Aeration: no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- Biomass loading rate: 25 Daphnia/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The resconstituted water medium used for testing was Elendts M4 Daphnia medium (Elendt and Bias (1990). Trace nutrient deficiency in Daphnia magna cultured in standard medium for toxicity testing. Effects of the optimisation of culture conditions on life history parameters of D.magna. Water Research )
- Total organic carbon: 12.4 mg/L
- Particulate matter: Total suspended solids 3.2 mg/L
- Chloride: 151.9 mg/L
- Alkalinity: 37.2 mg CaCO3/L
- Conductivity: 669uS/cm (at 25 degree C)

OTHER TEST CONDITIONS
- Photoperiod: 16h light and 8h dark with a 20 min. dawn/dusk transitional period.

EFFECT PARAMETERS MEASURED :
An assessment of the response of the Daphnia magna was made 24 and 48h after the start of the test. Each Daphnia was viewed by eye and was defined as affected if showing no whole body movement, relative to the water, within a 15 second period. These Daphnia were termed immobile. Any overt symptoms of toxcity were also recorded. The median effect concentration (EC50) was defined as the concentration resulting in 50% immobilisation of the Daphnia in the time period specified.

TEST CONCENTRATIONS
- Test concentrations: 120 mg/L of test substance and a dilution water control

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 3

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

120 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

There were no symptoms of toxicity observed in this study. None of the Daphnia were observed to be immobilised in the dilution water control and in the 120 mg/L test substance solution.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

It is concluded that as none of the Daphnia magna were observed as immobilised after 24 or 48h in the control or test concentration solutions and no other symptoms of toxicity were observed that the test substance has no toxic effect on Daphnia magna at 120 mg/L (based on nominal concentrations).

Executive summary:

Introduction

The study was performed to assess acute toxicity of the test substance to Daphnia magna. The method was designed to be compatible with the following guidelines:

OECD Guidelines for the testing of Chemicals (1993). Test guideline 202 Part I, Daphnia sp., Acute Immobilisation Test (1984)

Official Journal of the European Communities, L 383 A, Part C2, Acute toxicity for Daphnia (1992).

Results

After 48h no immobilisation was observed in the test substance test vessels or in the dilution water control.

Conclusion

The 24 and 48h EC50 was determined to be >120 mg/L of test substance. The 48h NOEC was 120 mg/L. The lowest nominal concentartion at which there was 100% immobilisation was >120 mg/L. It is concluded that as none of the Daphnia magna were observed as immobilised after 24 or 48h in the control or test concnetration solutions and no other symptoms of toxicity were observed that the test substance has no toxic effect on Daphnia magna at 120 mg/L (based on nominal concentrations).