Registration Dossier
Registration Dossier
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EC number: 605-350-3 | CAS number: 16397-70-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- p-Nitrobenzoic acid-2-ethylhexylester
- EC Number:
- 605-350-3
- Cas Number:
- 16397-70-9
- Molecular formula:
- C15 H21 N O4
- IUPAC Name:
- p-Nitrobenzoic acid-2-ethylhexylester
- Details on test material:
- - Name of test material (as cited in study report): p-Nitrobenzoesäure-2-2ethylhexylesther
- Molecular formula (if other than submission substance): C15H21NO4
- Molecular weight (if other than submission substance): 279
- Substance type: chemical
- Physical state: liquid
- Analytical purity: approx. 99.9 %
- Lot/batch No.: 3299/95/146 Fr 4A
- Storage condition of test material: room temperature
- water solubility: 1.4 mg/L
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- - Laboratory culture: yes
- Method of cultivation: laboratory STP
- Initial biomass concentration: 1 g/L dry mass
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 30 min
Test conditions
- Nominal and measured concentrations:
- nominal: 0, 1, 10, 100 mg/L
- Details on test conditions:
- Type: aquatic
TEST SYSTEM
- Test vessel: glass bottles
- Type (delete if not applicable): open / closed
- Material, size, headspace, fill volume: 250 ml
- Aeration: yes
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: OECD-medium
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : oxygen consumtion after 30 min
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1:10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
