2,2,3,3,8,8,9,9-octafluorotricyclo[8.2.2.2⁴,⁷]hexadeca-1(12),4,6,10,13,15-hexaene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

effects on growth of green algae

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Report date:

2022

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: OECD Series on Testing and Assessment No 23 - Guidance Document On Aquatic Toxicity Testing Of Difficult Substances And Mixtures ENV/JM/MONO(2019).

GLP compliance:

yes

Test material

Test material information

1

Reference substance name:

2,2,3,3,8,8,9,9-octafluorotricyclo[8.2.2.2⁴,⁷]hexadeca-1(12),4,6,10,13,15-hexaene

EC Number:

639-791-8

Cas Number:

3345-29-7

Molecular formula:

C16H8F8

IUPAC Name:

2,2,3,3,8,8,9,9-octafluorotricyclo[8.2.2.2⁴,⁷]hexadeca-1(12),4,6,10,13,15-hexaene

Specific details on test material used for the study:

Batch n. 340421
Production date November 19, 2021
Expiry date November 19, 2024
Purity ≥98.00%

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

yes

Remarks:

Due to low solubility of the test item in water, a solvent (acetonitrile) was used as carrier to add test substance in the test medium.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

23.8 – 24.1°C.

pH:

7.90 – 8.04 at the test start; 9.08 – 9.23 at the test end (mean values)

Nominal and measured concentrations:

negative control: algal medium without test item
solvent control: algal medium without test item but with acetonitrile solvent
1.0 mg/L test item.

Details on test conditions:

Light intensity: 5896 - 6680Lux.

Light regime: continuous illumination.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 3

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 4

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

yield

Effect concentrations 5

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

yield

Effect concentrations 6

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

yield

Effect concentrations 7

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 0.09 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Effect concentrations 8

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

0.09 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Effect concentrations 9

Duration:

72 h

Dose descriptor:

LOEC

Effect conc.:

> 0.09 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

No adverse effect was found after 72 hours of exposure at the nominal concentration of 1.0 mg PARYLENE HT® /L, corresponding to the maximum water solubility achieved in algal medium.
Since the test item concentration decreased during the exposure, the geometric mean of measured
concentration was calculated and was found equal to 0.09 mg PARYLENE HT®/L.

Executive summary:

A full dose-response test was performed to evaluate the effect of the test item on Pseudokirchneriella subcapitata.

Cell density was measured every 24 hours.

Microscopic observation was also performed to verify a normal and healthy appearance of the inoculum culture and to observe any abnormal appearance of the algae (as may be caused by the exposure to the test substance) at the end of the test.
No remarkable observations were made concerning the appearance of the cells of the inoculum culture and of the test item treated algae.

The analytical recoveries were referred to active ingredient nominal content (98.0%) declared on the Certificate of Analysis supplied by the Sponsor.

Since the analytical recoveries were not in the range 80 % - 120 % of the nominal concentrations during the test period, the biological results were referred to geometric mean of PARYLENE HT®
measured concentrations, as recommended by OECD Guideline No. 201.

 No adverse effect was found after 72 hours of exposure at the nominal concentration of 1.0 mgPARYLENE HT® /L, corresponding to the maximum water solubility achieved in algal medium.
Since the test item concentration decreased during the exposure, the geometric mean of measured
concentration was calculated and was found equal to 0.09 mg PARYLENE HT®/L.