Ethyl (2E,4Z)-2,4-decadienoate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan. 31-March 5, 2012

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl (2E,4Z)-2,4-decadienoate

EC Number:

221-178-8

EC Name:

Ethyl (2E,4Z)-2,4-decadienoate

Cas Number:

3025-30-7

Molecular formula:

C12H20O2

IUPAC Name:

ethyl deca-2,4-dienoate

Test material form:

not specified

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: VE00139176
- Expiration date of the lot/batch: April 27, 2013

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic (adaptation not specified)

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Bois-de-Bay, Satigny, Switzerland
- Storage conditions: aerobic
- Preparation of inoculum for exposure: The sludge was washed three times in mineral medium.
- Concentration of sludge: 5.46 g/L
- Water filtered: deionized water

Duration of test (contact time):

34 d

Initial test substance concentration

Initial conc.:

30 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Composition of medium: 50 mL of a 1 L solution containing 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4*2H2O, and 0.5 g NH4Cl, 5 mL of a 1 L solution containing 27.5 g CaCl2, 5 mL of a 1 L solution containing 22.5 g MgSO4*7H2O, and 5 mL of a 1 L solution containing 0.25 g FeCl3*6H2O, and one drop of concentrated HCl. 7 L of deionized water was also added.
- Test temperature: 22.1-23.5 degrees C
- pH: 7.4
- pH adjusted: yes
- Suspended solids concentration: 30 mg/L

TEST SYSTEM
- Culturing apparatus: Oxitop flasks
- Number of culture flasks/concentration: 2
- Measuring equipment: Oxitop flasks
- Details of trap for CO2 and volatile organics if used: Carbon dioxide is absorbed by sodium hydroxide pellets, and the resulting drop in pressure is measured.


SAMPLING
- Sampling frequency: The Oxitop system provides continuous automatic monitoring.
- Sampling method: Automated Oxitop system which provides continuous monitoring of pressure inside the flask without opening it.


CONTROL AND BLANK SYSTEM
- Inoculum blank: yes

STATISTICAL METHODS: mean

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

Test performance:

The reference substance degraded 40% after 7 days, and 65% after 14 days. No more than 20% difference between replicates were noted. The test is thus valid.

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

72

Sampling time:

28 d

BOD5 / COD results

Results with reference substance:

The reference substance degraded 40% after 7 days, and 65% after 14 days.

Any other information on results incl. tables

O₂ Uptake (mg O₂/L adjusted to nominal concentrations)

Day	Inoculum Blank (mean)	Test Substance (1)	Test Substance (2)	% Biodegradation of Test Substance (mean)
1	8.1	-0.4	-0.4	-1
2	14.2	21.4	21.4	27
12	29.6	50.8	52.2	66
14	31.7	53.4	53.3	68
21	35.7	58.7	55.4	73
28	42.4	57.6	54.8	72

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

The test substance biodegraded 72% in 28 days, and met the 10-day window requirement. It is therefore readily biodegradable.

Executive summary:

The biodegradability of the test substance was determined in an OECD Guideline 301F study. 30 mg/L of test substance was monitored for biodegradation by activated sludge for 34 days. Sodium benzoate was used as a reference substance. The oxygen consumption was monitored during this time. The reference substance results met the validity criteria. The test substance biodegraded 72% in 28 days, and met the 10-day window requirement.  It is therefore readily biodegradable.