Ethyl 2-bromopropionate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Ethyl 2-bromopropionate

EC Number:

208-609-5

EC Name:

Ethyl 2-bromopropionate

Cas Number:

535-11-5

Molecular formula:

C5H9BrO2

IUPAC Name:

ethyl 2-bromopropionate

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

Animals weigh 150-180 g and were fed and drunk ad libitum.
Animals were submitted to a water diet of 17-19 hours before receiving the treatment and feed/water.
Temperature of 22°C, hygrometry of 50% and ventilation of 8 air renewals/hour.

Administration / exposure

Route of administration:

oral: unspecified

Vehicle:

CMC (carboxymethyl cellulose)

Doses:

Preliminary test: 100, 500, 1000, 2500, 5000 mg/kg
Definitive test: 400, 440, 480, 580, 700 mg/kg

No. of animals per sex per dose:

Preliminary test: 2 rats/sex/dose
Definitive test: 5 rats/sex/dose

Control animals:

yes

Details on study design:

1 preliminary test and 1 definitive test

Statistics:

3 methods:
- Probit
- Litchfield and Wilcoxon
- Arcsinus

Results and discussion

Preliminary study:

At dose of 100 mg/kg, 0% of cumulated mortality after 14 days of observation.
At dose of 500 mg/kg, 50% of cumulated mortality after 14 days of observation.
At doses of 1000, 2500 and 5000 mg/kg, 100% of cumulated mortality after 14 days of observation.

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 439 mg/kg bw

Based on:

test mat.

95% CL:

>= 416 - <= 462

Remarks on result:

other: according to Litchfield and Wilcoxon method

Mortality:

At dose of 400 mg/kg, 20% of cumulated mortality after 14 days of observation.
At dose of 440 mg/kg, 50% of cumulated mortality after 14 days of observation.
At dose of 480 mg/kg, 80% of cumulated mortality after 14 days of observation.
At doses of 580 and 700 mg/kg, 100% of cumulated mortality after 14 days of observation.

Clinical signs:

other: At dose of 400 mg/kg: from 1h to 6h, reduced spontaneous activity, apathy, ptosis. At day 1, normal behavior. At doses of 440 and 480 mg/kg: from 1h to day 1, reduced/no spontaneous activity, apathy, ptosis. At day 2, persistence of apathy. At day 3, norm

Gross pathology:

Observations during autopsy of dead animals under test:
At dose of 400 mg/kg: hemorrhagic lungs and thymus
At doses of 440 and 480 mg/kg: irritated stomach, hemorrhagic lungs and thymus in most of the animals
At doses of 580 and 700 mg/kg: same kind of symptoms as above.

Observations during autopsy of surviving animals at the end of the test:
At dose of 480 mg/kg: pale kidneys. This anomaly was not observed at doses of 400 and 440 mg/kg.

Applicant's summary and conclusion

Interpretation of results:

Category 4 based on GHS criteria

Conclusions:

Under experimental conditions, the LD50 by oral route in rats of Ethyl-2-bromopropionate is 439 mg/kg (416-462) according to Litchfield and Wilcoxon method.