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Diss Factsheets

Administrative data

Endpoint:
sub-chronic toxicity: oral
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1984
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment

Data source

Reference
Reference Type:
publication
Title:
Toxicity of Trichlortoluene Isomers: A 28-Day Feeding Study in the Rat
Author:
I. Chu, S. Y. Shen, D. C. Villeneuve, V. E. Secours
Year:
1984
Bibliographic source:
J. Envirom. Sci. Health B19(2)

Materials and methods

Principles of method if other than guideline:
See below
GLP compliance:
no
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
2,6-dichlorobenzyl chloride
EC Number:
217-940-4
EC Name:
2,6-dichlorobenzyl chloride
Cas Number:
2014-83-7
Molecular formula:
C7H5Cl3
IUPAC Name:
2,6-dichlorobenzyl chloride
Details on test material:
purchades from Aldrich Chemical Co.

Test animals

Species:
rat
Strain:
Sprague-Dawley
Sex:
male/female
Details on test animals or test system and environmental conditions:
13 groups of 10 male/female each, acclimatized for one week, 12 h light/dark cycle, 20 +- 2 °C, 40 +-5% rh.

Administration / exposure

Route of administration:
oral: feed
Vehicle:
corn oil
Details on oral exposure:
Test substance was dissolved in corn oil and mixed with diet.
Analytical verification of doses or concentrations:
not specified
Duration of treatment / exposure:
28 days
Doses / concentrationsopen allclose all
Dose / conc.:
0 ppm
Dose / conc.:
0.5 ppm
Dose / conc.:
5 ppm
Dose / conc.:
50 ppm
Dose / conc.:
500 ppm
No. of animals per sex per dose:
10 male and female each
Control animals:
yes, concurrent vehicle
Positive control:
No

Examinations

Observations and examinations performed and frequency:
Body weight and food consumption were determined weekly, clinical observations made daily.
Sacrifice and pathology:
Gross examinations at necropsy
Other examinations:
Serum analysiss, hepathic mixed function oxidases

Results and discussion

Results of examinations

Clinical signs:
no effects observed
Mortality:
no mortality observed
Body weight and weight changes:
no effects observed
Food consumption and compound intake (if feeding study):
no effects observed
Food efficiency:
not examined
Water consumption and compound intake (if drinking water study):
not examined
Ophthalmological findings:
not examined
Haematological findings:
no effects observed
Clinical biochemistry findings:
effects observed, treatment-related
Description (incidence and severity):
At 500 ppm increades ADPM activities in male rats.
Urinalysis findings:
not examined
Behaviour (functional findings):
no effects observed
Immunological findings:
not examined
Organ weight findings including organ / body weight ratios:
no effects observed
Gross pathological findings:
effects observed, treatment-related
Description (incidence and severity):
Mild changes in observes organs liver, kidney, thyriod gland.
Liver: Mild regualr and irregular lobular patterns, heptatocytes had mild anisokaryosis associated with pyknosis, and occasionally necrotic hepatocytes were observed.
Kidney: The renal changes consisted of an accumulation of eosinophilic intracytoplysmic inclusion in the epithelium of proximal tubules associated with focal glomerular adhesions and interstitial scarring due to spontaneous aging process.
Thyroids: Reduction in follicular size and colloid densitiy. The epithelium cells became columnar and thickened with focal and multifocal angular collapse of follices. Focal and multifocal pypillars profilerations and focal vacuolations.
No residual substance in liver and fat at any dose.
Neuropathological findings:
not examined
Histopathological findings: non-neoplastic:
not examined
Histopathological findings: neoplastic:
not examined

Applicant's summary and conclusion

Conclusions:
The substance showed a low order of subacute oral toxixity in male and female rats. No accumulation in tissues due to exposure was observed.
Executive summary:

Groups of 10 male and 10 female rats were fed 2,6-dichlorobenzylchloride in their diet at 0, 0.5, 5. 50 and 500 ppm (corresponds to 0.048 - 46 mg/kg bw day in males and 0.053 - 53 mg/kg bw/day in females) for 28 days. Growth rate and food consumption were not affected by treatment. No deaths occured. Mild biochemical changes occured in male rats. Hepatic microsomal aminopyrine N-demethylase activities were increased in male rats at 500 ppm. Hematological parameters were not affected by treatment. Mild histological changes were seen in the liver, kidney and thyroid of treated rats. Data suggest that 2,6-dichlorobenzylchloride possesses a low order of oral toxicity in the rat.