Isopropyl laurate

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well- documtented study report which meets basic scientific principles (analytical purity of test substance is not specified).

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1977

Materials and methods

Principles of method if other than guideline:

The test samples are given at various concentrations by tail venapunction. Thereafter, the animals are observed for a 14 day period after which necropsies of survivors are carried out.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Isopropyl laurate

EC Number:

233-560-1

EC Name:

Isopropyl laurate

Cas Number:

10233-13-3

Molecular formula:

C15H30O2

IUPAC Name:

propan-2-yl dodecanoate

Details on test material:

- Name of test material (as cited in study report): isopropyllaurate
- Physical state: clear, colorless liquid
- Analytical purity: not given

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: from Institute's colony
- Weight at study initiation: male - 98 - 186g; female - 78 - 134g
- Housing: in groups of five in screenbottomed stainless steel cages
- Diet: stock diet, ad libitum
- Water: tap water, ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 23 - 25°C
- Air changes (per hr): weel-ventilated room

Administration / exposure

Route of administration:

intravenous

Vehicle:

propylene glycol

Doses:

2.88, 3.46, 4.15, 4.98 mL/kg bw (of a 10% dilution)
(2470, 2970, 3560, 4270 mg/kg bw - calculated using density (0.857 g/cm3) from hapter 4.4)

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: not specified
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

0.405 mL/kg bw

Based on:

test mat.

95% CL:

0.36 - 0.436

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

347 mg/kg bw

Based on:

other: calculated using density (0.857 g/cm3)

Mortality:

2.88 mL/kg - no animals died
3.46 mL/kg - 3 male and 1 female animal died
4.15 mL/kg - 3 male and 4 female animals died
4.98 mL/kg - 4 male and 4 female aninamls died

Clinical signs:

within one minute of dosing the animals showed convulsive movement of the hind legs, this disappaered after 10 to 30 seconds, thereafter, most rats showed short moments of aimless running, followed by loss of consciousness. Unconsciouness was observed for all treated animals, some recovered within 2 to 5 minutes after dosing, others died in this period by asphyxia.

Gross pathology:

no treatment related gross alterations

Other findings:

- Other observations: convulsive movements immediately after dosing were also observed in rats given propylene glycol