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EC number: 210-258-8 | CAS number: 611-19-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to aquatic algae and cyanobacteria
Administrative data
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- no data available
- Reliability:
- 1 (reliable without restriction)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 999
- Report date:
- 1999
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- α,2-dichlorotoluene
- EC Number:
- 210-258-8
- EC Name:
- α,2-dichlorotoluene
- Cas Number:
- 611-19-8
- Molecular formula:
- C7H6Cl2
- IUPAC Name:
- 1-chloro-2-(chloromethyl)benzene
- Details on test material:
- Source: Wako Pure Chemical Industries, Ltd.,
Purity 99.6 %,
Lot No. PAM5243
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
no data availble
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- no data available
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- Dispersant and Concentrations: Polyoxyethylenesorbitan fatty acid ester, 10 mg/l test solution
Test organisms
- Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- a) Strain Number: ATCC22662
b) Supplier/Source: American Type Culture Collection
c) Pretreatment: Subcultured for 3 days in OECD medium before use.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Post exposure observation period:
- no data available
Test conditions
- Hardness:
- no data available
- Test temperature:
- 23 +/- 2 °C
- pH:
- 7.9 - 8.0 at test start (0 hours)
8.0 - 10.5 after 72 h - Dissolved oxygen:
- no data available
- Salinity:
- no data available
- Nominal and measured concentrations:
- Nominal Concentrations (as mg/l): 0, 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10
- Details on test conditions:
- Medium: OECD medium (Table 1)
Exposure Vessel Type: 100-ml Medium in a 500-ml Conical Flask
Number of Replicates: 3
Initial Cell Number: 10,000 cells/ml
Light Condition: 4,000 - 5,000 lux, continuous - Reference substance (positive control):
- not specified
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.045 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.78 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
Any other information on results incl. tables
Measured concentrations at the beginning (0 hr) and the end
(72 hr) of the test were ranged within 66 - 91% and 19 - 43%
of the nominal concentrations, respectively. Because most of
the measured concentrations were lower than 80% of the
nominal, the time-weighted concentrations were used to
calculate effect values (Table 2).
Table 2.Measured concentrations of the test solutions in the
72-hour growth inhibition test on Selenastrum capricornutum
========================================================
Nominal Measured Time Measured/Nominal
concn. concn. -weighted (%)
(mg/l) (mg/l) mean
------------- ---------------
0 hr 72 hr (mg/l) 0 hr 72 hr
-------------------------------------------------------
Control <0.005 <0.005 --- --- ---
Solv. Control <0.005 <0.005 --- --- ---
0.10 0.0833 0.0201 0.0445 83 20
0.22 0.185 0.0419 0.0964 84 19
0.46 0.303 0.0983 0.182 66 21
1.0 0.912 0.427 0.639 91 43
2.2 1.59 0.591 1.01 72 27
4.6 3.49 1.15 2.11 76 25
10 8.13 2.32 4.63 81 23
========================================================
[Water Chemistry]
Table 3.The pH values of the test solutions in the 72-hour
Growth inhibition test on Selenastrum capricornutum.
======================================
Nominal concn. pH
--------------------
(mg/l) 0 hour 72 hours
--------------------------------------
Control 8.0 10.4
Solvent control 7.9 10.3
0.10 7.9 10.4
0.22 8.0 10.5
0.46 7.9 10.4
1.0 7.9 10.4
2.2 7.9 8.9
4.6 7.9 8.2
10 7.9 8.0
=======================================
[Effect Data]
Area method:
EbC50 (0 - 72 hr) = 0.78mg/l (Table 4 - 6)
NOEC (0 - 72 hr) = 0.045 mg/l (Table4, 5 & 7)
Rate method:
ErC50 (0 - 24 hr) = 1.5 mg/l
(95% confidence limits: 1.2 - 1.9 mg/l) (Table 4 - 6)
NOEC (0 - 24 hr) = 0.096 mg/l (Table4, 5 & 7)
ErC50 (24 - 48 hr) = 1.2 mg/l
(95% confidence limits: 1.16 - 1.21 mg/l) (Table 4 - 6)
NOEC (24 - 48 hr) = 0.64 mg/l (Table4, 5 & 7)
ErC50 (24-72hr) = 1.2 mg/l
(95% confidence limits: 1.1 - 1.3 mg/l) (Table 4 - 6)
NOEC (24 - 72 hr) = 0.18 mg/l (Table4, 5 & 7)
Table 4.Mean cell concentrations and their standard
deviations (S. D.) in the test cultures and controls
=======================================================
Nominal concn Cell concentration (x 10000 cells/ml)
(mg/l) 0 hr 24 hr 48 hr 72 hr
-------------------------------------------------------
Control Mean 1.0 6.98 34.51 89.12
S. D. 0.0 0.28 1.37 0.65
-------------------------------------------------------
Solvent control Mean 1.0 7.19 37.96 90.52
S. D. 0.0 0.23 2.91 3.79
-------------------------------------------------------
0.10 Mean 1.0 6.82 35.32 85.47
S. D. 0.0 0.45 1.07 4.37
-------------------------------------------------------
0.22 Mean 1.0 6.49 33.39 76.51
S. D. 0.0 0.37 3.08 6.09
-------------------------------------------------------
0.46 Mean 1.0 5.70 30.85 65.81
S. D. 0.0 0.24 0.98 0.91
-------------------------------------------------------
1.0 Mean 1.0 5.58 28.18 61.64
S. D. 0.0 0.37 2.38 4.74
-------------------------------------------------------
2.2 Mean 1.0 2.73 7.23 17.51
S. D. 0.0 0.11 0.30 1.01
-------------------------------------------------------
4.6 Mean 1.0 1.90 2.00 2.17
S. D. 0.0 0.06 0.06 0.10
-------------------------------------------------------
10 Mean 1.0 1.55 1.59 1.58
S. D. 0.0 0.05 0.03 0.08
=======================================================
Table 5.Percent inhibition of the cell growth (IA) and the
average specific growth rates (Im) in the 72-hour growth
inhibition test on Selenastrum capricornutum
========================================================
Nominal Area Inhibition Rate Inhibition
concn. A IA (%) M Im (%)
--------------------- --------------------
(mg/l) (0-72 hr) 0-24 hr)
--------------------------------------------------------
Control 20050800 --- 0.080918 ---
Solv. cont. 21099600 -5.23 0.082181 -1.56
0.10 19769600 1.40 0.079954 1.19
0.22 18151600 9.47 0.077859 3.78
0.46 16068400 19.86 0.072471 10.44
1.0 14899600 25.69 0.071574 11.55
2.2 3891600 80.59 0.041823 48.31
4.6 596400 97.03 0.026732 66.96
10 343600 98.29 0.018244 77.45
========================================================
Table 5.continue
=======================================================
Nominal Rate Inhibition Rate Inhibition
concn. M Im (%) M Im (%)
--------------------- -------------------
(mg/l) (24-48 hr) (24-72 hr)
-------------------------------------------------------
Control 0.066612 --- 0.053081 ---
Solv. cont. 0.069260 -3.98 0.052764 0.60
0.10 0.068548 -2.91 0.052675 0.77
0.22 0.068197 -2.38 0.051387 3.19
0.46 0.070396 -5.68 0.050988 3.94
1.0 0.067441 -1.24 0.050032 5.75
2.2 0.040579 39.08 0.038706 27.08
4.6 0.002138 96.79 0.002760 94.80
10 0.001073 98.39 0.000436 99.18
========================================================
Table 6.Calculated EC50 values
========================================================
Value 95% confidence limits Ordinate
(mg/l) (mg/l)
--------------------------------------------------------
EbC50 (0-72 hr) 0.78 not calculated IA
ErC (0-24 hr) 1.5 1.2 - 1.9 Im
ErC (24-48 hr) 1.2 1.16 - 1.21 Im
ErC (24-72 hr) 1.2 1.1 - 1.3 Im
========================================================
Table 7.Calculated NOEC
========================================================
Value Statistical Parameter
(mg/l) method
--------------------------------------------------------
NOEC (0-72 hr) 0.045 Dunnett (p<0.05) IA
NOEC (0-24 hr) 0.096 Dunnett (p<0.05) Im
NOEC (24-48 hr) 0.64 Dunnett (p<0.05) Im
NOEC (24-72 hr) 0.18 Dunnett (p<0.05) Im
========================================================
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 72 hour EbC50 of Selenastrum capricornutum was determiend to be 0.78 mg/L OCBC.
- Executive summary:
OCBC was assessed in a chronic toxicity test to Selenastrum capricornutum, according to OECD guideline 201. Algae were exposed in a static test to the test item for 72 hours at nominal concentrations of 0.10, 0.22, 0.46, 1.0, 2.2, 4.6 and 10.0 mg/L. The 72 h EbC50 was determined to be 0.78 mg/L.
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